| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SLD5 |
| WB Predicted band size | 26 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GINS4抗体的3篇文献示例(注：以下内容为模拟概括，具体文献需根据实际检索结果调整)：
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1. **文献名称**: *GINS4 promotes cell proliferation in colorectal cancer via DNA replication regulation*
**作者**: Zhang Y, et al. (2015)
**摘要**: 该研究通过免疫组化和Western blot技术分析GINS4在结直肠癌组织中的表达，发现其过表达与肿瘤增殖和不良预后相关。研究使用特异性GINS4抗体证实其在DNA复制中的调控作用，并提示其作为潜在治疗靶点的可能性。
2. **文献名称**: *Structural insights into the GINS complex assembly mediated by GINS4 subunit*
**作者**: Chunhui Zhou, et al. (2009)
**摘要**: 本研究解析了GINS复合体的结构，重点探讨GINS4亚基在复合体稳定性中的作用。通过免疫共沉淀(Co-IP)结合GINS4抗体，揭示了其与PSF1、PSF2和PSF3的相互作用机制，为理解DNA复制起始提供分子基础。
3. **文献名称**: *GINS4 as a biomarker for hepatocellular carcinoma progression*
**作者**: Li X, et al. (2018)
**摘要**: 利用GINS4抗体对肝癌患者组织样本进行检测，发现GINS4高表达与肿瘤分期和转移显著相关。体外实验通过siRNA敲低GINS4抑制肝癌细胞增殖，表明其可作为肝癌诊断和预后的潜在标志物。
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如需具体文献，建议在PubMed或Web of Science中检索关键词“GINS4 antibody”或“GINS4 function”，并筛选涉及抗体应用(如Western blot、免疫组化)或机制研究(如DNA复制、癌症关联)的论文。
The GINS4 antibody targets the GINS complex subunit 4 (GINS4), a critical component of the evolutionarily conserved GINS complex, which plays a vital role in DNA replication. The GINS complex, comprising GINS1. GINS2. GINS3. and GINS4. is essential for the initiation and elongation phases of replication. It interacts with the CDC45 protein and the MCM helicase to form the CMG (CDC45-MCM-GINS) complex, enabling unwinding of double-stranded DNA and ensuring replication fork progression. GINS4 stabilizes the GINS complex, facilitating its recruitment to replication origins and maintaining genomic integrity.
Antibodies against GINS4 are widely used to study DNA replication mechanisms, cell cycle regulation, and replication stress responses. They are employed in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess GINS4 expression, localization, and interactions. Dysregulation of GINS4 has been linked to cancers, as its overexpression is observed in tumors, correlating with proliferation and poor prognosis. Research using GINS4 antibodies helps elucidate its role in oncogenesis and potential as a therapeutic target. These antibodies are validated for specificity, often through knockdown/knockout models, ensuring reliability in experimental and diagnostic contexts.
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