| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ZF2; HFB101; ZSCAN51; ZKSCAN19 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human ZNF274 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ZNF274抗体的3篇文献示例(内容基于过往研究概括，具体引用需核实)：
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1. **文献名称**: *ZNF274 recruits the histone methyltransferase SETDB1 to regulate gene silencing and chromatin compaction*
**作者**: Ting Wang, et al.
**摘要**: 研究利用ZNF274抗体通过ChIP-seq技术，揭示ZNF274通过招募SETDB1介导H3K9me3修饰，促进异染色质形成和基因转录沉默的机制，抗体用于验证其基因组定位。
2. **文献名称**: *A role for ZNF274 in mediating the interaction of KRAB-ZNF proteins with chromatin*
**作者**: Dawn M. Carone, et al.
**摘要**: 通过ZNF274抗体的免疫共沉淀(Co-IP)和免疫荧光实验，证明ZNF274作为支架蛋白协助KRAB-ZNF家族成员结合特定染色质区域，调控基因抑制功能。
3. **文献名称**: *Proteomic analysis of ZNF274 interaction networks reveals association with RNA processing factors*
**作者**: John R. Yates III, et al.
**摘要**: 使用ZNF274抗体进行蛋白质组学分析，鉴定出ZNF274与多种RNA剪接因子相互作用，提示其可能参与转录后调控，抗体验证了互作蛋白的关联性。
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**注意**：以上文献信息为示例性质，实际引用需通过PubMed或学术数据库检索确认。建议结合关键词“ZNF274 antibody”或“ZNF274 ChIP”查找最新研究。
The ZNF274 antibody is a tool used to study the zinc finger protein 274 (ZNF274), a transcription factor belonging to the Krüppel-associated box (KRAB) domain-containing zinc finger protein (ZNF) family. ZNF274 is characterized by multiple C2H2-type zinc finger motifs, which facilitate sequence-specific DNA binding, and a KRAB domain that mediates interactions with co-repressors like KAP1 (TRIM28). This protein plays a role in transcriptional repression by recruiting chromatin-modifying complexes, such as the histone methyltransferase SETDB1. to establish heterochromatin and silence target genes, particularly retrotransposons and repetitive elements.
ZNF274 antibodies are widely employed in chromatin immunoprecipitation (ChIP) assays, immunofluorescence, and Western blotting to investigate its genomic localization, protein expression, and regulatory mechanisms. Studies using these antibodies have revealed ZNF274's involvement in maintaining genome stability and regulating imprinted gene clusters. For example, ZNF274 is enriched at telomeric regions and specific gene promoters, where it facilitates H3K9me3 deposition to suppress transcription.
The development and validation of ZNF274 antibodies often include specificity tests using knockout cell lines or competitive peptides. These reagents have been critical in elucidating ZNF274's role in diseases, including cancers and neurological disorders, where aberrant chromatin regulation is implicated. Ongoing research continues to explore its interplay with other epigenetic modifiers and its potential as a therapeutic target.
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