| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MMP-7, MPSL1, PUMP-1 |
| WB Predicted band size | 30 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human MMP7 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于ZNF302抗体的模拟参考文献示例(请注意，这些文献为虚构示例，实际文献需通过学术数据库查询)：
---
1. **文献名称**: "Development and Application of a Monoclonal Antibody for ZNF302 in Embryonic Stem Cells"
**作者**: Smith A, et al.
**摘要**: 本研究开发了一种特异性识别ZNF302蛋白的单克隆抗体，通过免疫印迹和免疫荧光验证其在人胚胎干细胞中的表达。研究发现ZNF302通过调控多能性基因(如OCT4)参与早期胚胎发育。
2. **文献名称**: "ZNF302 Antibody-Based ChIP-seq Reveals Its Role in Chromatin Remodeling"
**作者**: Zhang L, et al.
**摘要**: 利用ZNF302抗体进行染色质免疫沉淀测序(ChIP-seq)，发现其在多能干细胞中结合特定DNA序列，调控染色质重塑和细胞分化，提示其可能通过表观遗传机制维持干细胞特性。
3. **文献名称**: "A Polyclonal Antibody Against ZNF302 Identifies Its Tumor-Suppressive Function in Colorectal Cancer"
**作者**: Lee J, et al.
**摘要**: 通过兔多克隆抗体检测ZNF302在结直肠癌组织中的表达，发现其低表达与肿瘤转移相关。功能实验表明ZNF302通过抑制上皮-间质转化(EMT)发挥抑癌作用。
4. **文献名称**: "Comparative Analysis of ZNF302 Antibodies for Epigenetic Studies"
**作者**: Johnson R, et al.
**摘要**: 本研究评估了多种商业ZNF302抗体的特异性，优化了其在免疫组化中的应用，并强调抗体选择对研究ZNF302在神经发育疾病中表达异常的重要性。
---
**注意**：以上内容为模拟示例，实际文献请通过PubMed、Google Scholar等平台以关键词“ZNF302 antibody”或“ZNF302 immunohistochemistry”检索。若研究较少，可扩展至ZNF蛋白家族或表观调控相关领域。
The ZNF302 antibody is a tool used to study the zinc finger protein 302 (ZNF302), a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family. ZNF302 functions as a transcriptional repressor by binding to specific DNA sequences through its C2H2-type zinc finger motifs, which mediate sequence recognition, and its KRAB domain, which recruits co-repressors like KAP1 to establish heterochromatin and silence target genes. This protein is implicated in regulating cellular processes such as differentiation, proliferation, and apoptosis. Studies suggest its involvement in embryonic development and tissue homeostasis, though its exact biological roles remain under investigation.
Antibodies against ZNF302 are primarily utilized in research to detect and quantify the protein in various experimental models, including immunohistochemistry (IHC), Western blotting, and immunofluorescence. These antibodies help elucidate ZNF302's expression patterns, subcellular localization (typically nuclear), and interactions with chromatin or other regulatory complexes. Notably, ZNF302 has been linked to cancer biology, where it may act as either an oncogene or tumor suppressor depending on cellular context, modulating pathways like Wnt/β-catenin or p53. Dysregulation of ZNF302 has been observed in malignancies such as hepatocellular carcinoma and gliomas. The antibody thus serves as a critical reagent for exploring ZNF302's role in epigenetic regulation, disease mechanisms, and potential therapeutic targeting. Commercial ZNF302 antibodies are typically validated for specificity using knockout/knockdown controls.
×
