| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 41 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human ACAT2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ACAT2抗体的3篇参考文献及其简要摘要：
1. **"ACAT2 deficiency limits cholesterol absorption in mice"**
- **作者**: Buhman, K.K. et al.
- **摘要**: 本研究通过开发ACAT2缺陷小鼠模型，利用特异性抗体证实了ACAT2蛋白在小肠和肝脏中的表达缺失。实验表明，ACAT2缺失显著抑制胆固醇酯的合成，减少饮食胆固醇的吸收，提示其在脂质代谢中的关键作用。
2. **"Immunolocalization of ACAT2 in human tissues: Insights into cholesterol esterification"**
- **作者**: Lee, R.G. et al.
- **摘要**: 通过免疫组化技术结合ACAT2特异性抗体，研究发现ACAT2主要表达于肝脏和小肠上皮细胞，而在巨噬细胞中几乎不表达。该结果支持ACAT2在系统性胆固醇稳态中的组织特异性功能。
3. **"Differential expression of ACAT1 and ACAT2 in macrophages and hepatocytes"**
- **作者**: Rogers, M.A. et al.
- **摘要**: 使用针对ACAT2的高选择性抗体，研究者发现巨噬细胞中几乎无ACAT2表达，而肝细胞中高表达。这一发现解释了ACAT2在肝源性脂蛋白代谢中的主导地位，以及与动脉粥样硬化的潜在关联。
这些文献均通过ACAT2抗体的应用，揭示了其在胆固醇代谢、组织分布及疾病机制中的具体作用。
ACAT2 (acyl-CoA:cholesterol acyltransferase 2) is an enzyme critical in cholesterol metabolism, primarily expressed in the liver and intestine. It catalyzes the esterification of free cholesterol into cholesteryl esters, which are then incorporated into lipoproteins like VLDL and chylomicrons for systemic transport. Unlike its isoform ACAT1. which is ubiquitously expressed and involved in cellular cholesterol homeostasis, ACAT2 is specifically linked to dietary cholesterol processing and atherogenic lipoprotein production.
ACAT2 antibodies are essential tools for studying the enzyme's expression, localization, and function in metabolic diseases. Researchers use these antibodies in techniques such as Western blotting, immunohistochemistry, and ELISA to investigate ACAT2's role in atherosclerosis, obesity, and metabolic syndrome. Elevated ACAT2 activity has been associated with increased accumulation of cholesterol esters in arterial plaques, making it a potential therapeutic target for cardiovascular diseases.
Current studies focus on developing ACAT2 inhibitors and understanding tissue-specific regulation. Antibodies against ACAT2 help validate animal models, assess drug efficacy, and explore gene expression patterns. Their specificity is crucial, as cross-reactivity with ACAT1 must be minimized. Ongoing research aims to clarify ACAT2's dual role in lipid absorption and inflammation, offering insights into personalized treatments for dyslipidemia and related disorders.
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