| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | XPB; BTF2; Ssl2; TTD2; GTF2H; RAD25; TFIIH |
| WB Predicted band size | 89 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human ERCC3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ERCC3抗体的3篇参考文献示例(文献信息为虚构，仅供示例用途)：
1. **文献名称**: "ERCC3/XPB Antibody Validation in DNA Repair Studies"
**作者**: Smith A, et al.
**摘要**: 该研究验证了一种高特异性ERCC3抗体的应用，证实其在免疫印迹(Western blot)和免疫荧光中能有效识别内源性ERCC3蛋白。研究发现ERCC3在紫外线诱导的DNA损伤修复中动态定位至细胞核损伤位点，支持其在核苷酸切除修复(NER)中的关键作用。
2. **文献名称**: "ERCC3 Expression Correlates with Chemotherapy Resistance in Ovarian Cancer"
**作者**: Lee H, et al.
**摘要**: 通过免疫组织化学(IHC)分析卵巢癌组织样本，发现ERCC3蛋白的高表达与铂类化疗耐药性显著相关。研究提示ERCC3可能作为预测化疗疗效的生物标志物。
3. **文献名称**: "Role of ERCC3 in Transcription-Coupled Repair: Insights from Knockdown Models"
**作者**: Garcia R, et al.
**摘要**: 利用特异性抗体检测ERCC3蛋白在转录偶联修复(TCR)中的功能，发现其与RNA聚合酶II的相互作用在修复活性氧损伤中至关重要。研究揭示了ERCC3在维持基因组稳定性中的双重角色(修复与转录)。
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如需真实文献，建议通过PubMed或Google Scholar以“ERCC3 antibody”、“XPB antibody”或“DNA repair antibody applications”为关键词检索近年研究。
The ERCC3 (Excision Repair Cross-Complementation Group 3) antibody is a tool used to detect the ERCC3 protein, a critical component of the nucleotide excision repair (NER) pathway responsible for correcting DNA damage caused by UV radiation and chemical adducts. ERCC3. also known as XPB, functions as a DNA helicase within the TFIIH (transcription factor IIH) complex, playing dual roles in DNA repair and transcription initiation. It unwinds DNA around lesion sites during NER and assists RNA polymerase II in promoter escape during transcription.
Mutations in the ERCC3 gene are linked to xeroderma pigmentosum (XP) complementation group B, a rare autosomal recessive disorder characterized by extreme UV sensitivity, predisposition to skin cancers, and neurological abnormalities. ERCC3 defects are also associated with combined features of XP and Cockayne syndrome (CS), highlighting its broader role in genome stability.
ERCC3 antibodies are widely used in research to study DNA repair mechanisms, transcription regulation, and disease pathology. They enable detection of ERCC3 expression via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Such studies help elucidate its interaction partners, cellular localization, and dysregulation in cancer or aging-related disorders. Additionally, these antibodies may have diagnostic potential in identifying ERCC3 mutations or assessing therapeutic responses in precision oncology.
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