首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠载脂蛋白C-II(APOC2)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APOC2含量。APOC2即载脂蛋白C2，它在脂质代谢中发挥重要作用，是脂蛋白脂肪酶（LPL）的激活剂，可促进乳糜微粒和极低密度脂蛋白的分解代谢。目前关于APOC2的研究，多围绕其在脂质代谢异常相关疾病如高脂血症等中的机制，探索其作为治疗靶点的潜力。试剂盒检测范围为18.8 ng/mL-1200 ng/mL，科研人员可通过研究脂代谢异常相关疾病（如肥胖、糖尿病）的分子机制，或评估药物干预对脂代谢途径的影响；为心血管疾病及代谢综合征的基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Apoc2Apolipoprotein C-II ELISA kit; Apo-CII ELISA kit; ApoC-II ELISA kit; Apolipoprotein C2 ELISA kit
缩写：APOC2
Uniprot No.：Q05020
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：18.8 ng/mL-1200 ng/mL
灵敏度：9.4 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse APOC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 97 | ||||
| Range % | 88-102 | |||||
| 1:40 | Average % | 93 | ||||
| Range % | 85-98 | |||||
| 1:80 | Average % | 103 | ||||
| Range % | 96-107 | |||||
| 1:160 | Average % | 104 | ||||
| Range % | 95-109 | |||||
回收率：
| The recovery of mouse APOC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-99 | ||||
| EDTA plasma (n=4) | 101 | 94-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 12000.0940.0970.096 6000.2290.2210.225 3000.4290.4440.437 1500.7070.7260.717 751.1091.0681.089 37.51.5351.4741.505 18.81.6821.6391.661 02.0481.9672.008 | ||||||
货期：3-5 working days
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