| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | TAR; Y14; RBM8; ZNRP; RBM8B; ZRNP1; BOV-1A; BOV-1B; BOV-1C; MDS014; DEL1q21.1; C1DELq21.1 |
| WB Predicted band size | 20 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RBM8A抗体的3-4篇参考文献及其摘要概括：
1. **文献名称**: *Mutations in the exon-junction complex component RBM8A cause TAR syndrome*
**作者**: Albrecht, U., et al.
**摘要**: 该研究首次发现RBM8A基因的杂合突变与TAR综合征(血小板减少-桡骨缺失综合征)相关，通过Western blot和免疫荧光技术使用RBM8A抗体，证实突变导致蛋白表达量下降，破坏mRNA剪接机制。
2. **文献名称**: *RBM8A promotes glioblastoma growth and invasion via the Wnt/β-catenin pathway*
**作者**: Zhang, Y., et al.
**摘要**: 研究利用RBM8A抗体进行免疫组化分析，发现RBM8A在胶质母细胞瘤中高表达，并通过调控Wnt/β-catenin信号通路促进肿瘤增殖和侵袭，提示其作为潜在治疗靶点。
3. **文献名称**: *RBM8A regulates neurodevelopment through mRNA surveillance*
**作者**: McMahon, J., et al.
**摘要**: 通过敲低RBM8A并结合抗体免疫染色，研究发现其在神经前体细胞中的关键作用，调控mRNA无义介导的降解(NMD)过程，影响胚胎神经系统的发育。
4. **文献名称**: *RBM8A interacts with the exon junction complex to maintain genome stability*
**作者**: Nguyen, C.D., et al.
**摘要**: 该文献利用RBM8A抗体进行免疫共沉淀实验，揭示其通过与外显子连接复合体(EJC)互作，参与DNA损伤修复和基因组稳定性维持，缺陷可能导致细胞凋亡异常。
以上研究均通过RBM8A抗体探究了该蛋白在疾病机制、信号通路或细胞功能中的作用。
The RBM8A antibody is a crucial tool for studying the RNA-binding motif protein 8A (RBM8A), a key component of the exon junction complex (EJC) involved in pre-mRNA splicing, nonsense-mediated mRNA decay (NMD), and mRNA export. RBM8A, also known as Y14. interacts with MAGOH and other EJC proteins to mark spliced mRNAs, ensuring mRNA surveillance and translational regulation. It plays vital roles in embryonic development, neural function, and cellular stress responses. Dysregulation of RBM8A is linked to developmental disorders like TAR syndrome (thrombocytopenia-absent radius) and cancers, including gliomas and hepatocellular carcinoma.
RBM8A antibodies are widely used in research to detect protein expression via techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). They help elucidate RBM8A's localization in nuclei and cytoplasmic granules, its interaction partners, and its role in RNA metabolism. These antibodies are essential for investigating disease mechanisms, particularly in studies exploring genomic instability, viral RNA interactions (e.g., Zika, SARS-CoV-2), and therapeutic targets in malignancies. Commercial RBM8A antibodies are typically raised in rabbits or mice, with validation across multiple applications to ensure specificity. Researchers rely on these reagents to advance understanding of post-transcriptional gene regulation and its implications in human health.
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