首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠载脂蛋白A-IV(APOA4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APOA4含量。APOA4 是一个重要靶点。它在脂质代谢、心血管疾病等方面有重要作用。其研究机制主要聚焦于它在脂蛋白合成与代谢中的功能，以及它对炎症反应、氧化应激的调节等，探索其与疾病发生发展关联，为相关疾病治疗提供思路。试剂盒检测范围为9.38 ng/mL-600 ng/mL，适用于肥胖、高脂血症、动脉粥样硬化等代谢性疾病模型的机制研究，也可用于评估药物或饮食干预对脂代谢通路的影响，满足心血管、内分泌等领域的科研需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Apoa4Apolipoprotein A-IV ELISA kit; Apo-AIV ELISA kit; ApoA-IV ELISA kit; Apolipoprotein A4 ELISA kit
缩写：APOA4
Uniprot No.：P02651
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：9.38 ng/mL-600 ng/mL
灵敏度：2.34 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat APOA4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 93 | ||||
| Range % | 87-97 | |||||
| 1:400 | Average % | 101 | ||||
| Range % | 97-105 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:1600 | Average % | 95 | ||||
| Range % | 91-101 | |||||
回收率：
| The recovery of rat APOA4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 89 | 83-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected6002.5672.6152.5912.4543001.9342.0782.0061.8691501.3241.3971.3611.224750.8310.8220.8270.69037.50.5810.5740.5780.44118.750.3340.3230.3290.1929.380.2610.2680.2650.12800.1360.1370.137 | ||||||
货期：3-5 working days
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