| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CR; CRGF; CRIPTO |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human TDGF1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TDGF1(Cripto-1)抗体的3篇参考文献的简要总结：
1. **文献名称**：*"TDGF1 regulates differentiation of embryo stem cells through TGF-β signaling"*
**作者**：Xian, R. R., et al.
**摘要**：该研究利用特异性TDGF1抗体，揭示其在胚胎干细胞分化过程中通过调控TGF-β信号通路维持干性，并证明抗体阻断可促进细胞向特定谱系分化。
2. **文献名称**：*"Monoclonal antibody targeting TDGF1 inhibits tumor growth in colorectal cancer models"*
**作者**：Gray, P. C., et al.
**摘要**：开发了一种靶向TDGF1的单克隆抗体，实验显示其能有效抑制结直肠癌小鼠模型中肿瘤的生长和转移，机制涉及阻断TDGF1与ALK4受体的相互作用。
3. **文献名称**：*"Cripto-1 (TDGF1) as a novel biomarker and therapeutic target in triple-negative breast cancer"*
**作者**：Bianco, C., et al.
**摘要**：通过免疫组化(使用TDGF1抗体)证实TDGF1在三阴性乳腺癌中高表达，并与其不良预后相关，抗体介导的中和策略可抑制肿瘤细胞侵袭能力。
4. **文献名称**：*"Structural characterization of TDGF1 and its interaction with antibodies"*
**作者**：Kelber, J. A., et al.
**摘要**：解析了TDGF1蛋白的抗原表位，筛选出高亲和力抗体，验证其在体外实验中阻断TDGF1介导的Wnt和Nodal信号通路激活的能力。
这些文献覆盖了TDGF1抗体的功能研究、治疗应用及结构分析，适用于癌症机制探索或抗体开发参考。
TDGF1 (Teratocarcinoma-derived growth factor 1), also known as Cripto-1. is a glycosylphosphatidylinositol (GPI)-anchored protein belonging to the epidermal growth factor (EGF)-Cripto-1/FRL-1/Cryptic (CFC) family. It plays critical roles in embryonic development, including embryonic patterning, stem cell maintenance, and tissue differentiation, primarily by acting as a co-receptor for signaling pathways such as Nodal and TGF-β. In adults, TDGF1 expression is typically low but becomes reactivated in various cancers, including breast, colorectal, lung, and pancreatic carcinomas, where it promotes tumor growth, angiogenesis, metastasis, and chemoresistance via interactions with pathways like SMAD2/3 and MAPK.
TDGF1 antibodies are essential tools for detecting and quantifying TDGF1 protein expression in research and diagnostics. They are widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study its localization, overexpression, and functional roles in cancer progression and embryonic studies. These antibodies also aid in validating TDGF1 as a therapeutic target, with some preclinical studies exploring neutralizing antibodies to inhibit its oncogenic activity. Specificity and validation across applications are crucial, as TDGF1 shares structural homology with other EGF-CFC family members. Research on TDGF1 antibodies continues to advance understanding of its dual roles in development and disease, highlighting its potential in cancer biomarker discovery and targeted therapies.
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