首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠载脂蛋白B48(apo-B48)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的apo-B48含量。apo-B48是重要的血脂相关靶点。其背景在于它是乳糜微粒的主要载脂蛋白，在脂质代谢中起关键作用。研究机制主要围绕调控其合成、分泌等环节，以降低血浆中富含甘油三酯脂蛋白水平，为高脂血症等疾病治疗提供新方向。试剂盒检测范围为62.5 ng/mL-4000 ng/mL，适用于研究脂代谢相关动物模型中apo - B48的动态变化，例如高脂饮食诱导的肥胖小鼠、肠道功能缺陷模型或基因编辑动物；为探究脂蛋白代谢调控机制、药物干预效果评估等基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：apo-B48
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 ng/mL-4000 ng/mL
灵敏度：15.6 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse apo-B48 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 96 | ||||
| Range % | 92-104 | |||||
| 1:400 | Average % | 93 | ||||
| Range % | 89-97 | |||||
| 1:800 | Average % | 87 | ||||
| Range % | 84-92 | |||||
| 1:1600 | Average % | 87 | ||||
| Range % | 82-93 | |||||
回收率：
| The recovery of mouse apo-B48 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-99 | ||||
| EDTA plasma (n=4) | 98 | 94-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 40000.0730.0740.074 20000.1170.1200.119 10000.1830.1920.188 5000.2830.2750.279 2500.4510.4480.450 1250.7700.7680.769 62.51.0481.1221.085 02.2752.3712.323 | ||||||
货期：3-5 working days
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