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产品描述：猪载脂蛋白E(Apo-E)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APOE含量。APOE 即载脂蛋白 E，在脂质代谢、神经保护等方面起关键作用。其基因多态性与阿尔茨海默病、心血管病等密切相关。研究机制包括影响脂质转运、调节免疫反应、参与神经细胞间信号传导，为相关疾病的诊断和治疗提供了重要靶点。试剂盒检测范围为31.25 ng/mL-2000 ng/mL，本产品适用于科研领域中对猪脂代谢机制、营养干预模型或相关病理生理研究的Apo - E水平分析，为动物实验样本提供稳定、特异的检测方案本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：APOEApolipoprotein E ELISA kit; Apo-E ELISA kit
缩写：APOE
Uniprot No.：P18650
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 ng/mL-2000 ng/mL
灵敏度：7.81 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 96 | |||||
| Range % | 85-105 | ||||||
| 1:200 | Average % | 90 | |||||
| Range % | 89-98 | ||||||
| 1:400 | Average % | 96 | |||||
| Range % | 82-103 | ||||||
| 1:800 | Average % | 97 | |||||
| Range % | 92-99 | ||||||
回收率：
| The recovery of pig Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 97 | 92-105 | |||||
| EDTA plasma (n=4) | 95 | 85-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2Average 20000.1310.1340.133 10000.2230.2260.225 5000.3350.3410.338 2500.5620.5530.558 1250.8620.8600.861 62.51.1921.2141.203 31.251.5891.6071.598 02.4172.3952.406 | |||||||
货期：3-5 working days
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