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Rabbit Polyclonal ANKRD27 Antibody

  • 中文名: ANKRD27抗体
  • 别    名: VARP; PP12899
货号: IPDX09280
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/25-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

AliasesVARP; PP12899
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenFusion protein of human ANKRD27
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于ANKRD27抗体的示例性参考文献(注:以下内容为基于领域知识的合理推测,可能非真实文献,建议通过学术数据库检索具体研究):

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1. **文献名称**: *ANKRD27 interacts with clathrin to regulate endosomal trafficking*

**作者**: Smith JL, et al.

**摘要**: 研究揭示了ANKRD27通过其C端结构域与网格蛋白结合,调控早期内体运输过程。通过免疫共沉淀和siRNA敲低实验,发现ANKRD27缺失导致细胞膜受体回收异常,提示其在细胞内运输中的关键作用。

2. **文献名称**: *ANKRD26 and ANKRD27 genetic variants in familial Parkinson's disease*

**作者**: Garcia-Ruiz S, et al.

**摘要**: 该研究分析了ANKRD27基因突变与帕金森病的关联。利用ANKRD27抗体进行免疫组化,发现突变体蛋白在患者神经元中异常聚集,提示其可能参与神经退行性疾病的病理机制。

3. **文献名称**: *Expression profiling of ANKRD27 in colorectal cancer and its prognostic significance*

**作者**: Chen H, et al.

**摘要**: 通过免疫印迹和组织芯片技术,研究发现ANKRD27在结直肠癌组织中高表达,且与患者生存率负相关。实验表明ANKRD27可能通过促进肿瘤细胞迁移参与癌症进展。

4. **文献名称**: *Characterization of a novel polyclonal antibody against ANKRD27 for subcellular localization studies*

**作者**: Müller R, et al.

**摘要**: 本文报道了一种新型ANKRD27多克隆抗体的开发与验证。通过免疫荧光和流式细胞术,证实该抗体可特异性识别内源性ANKRD27蛋白,并揭示其在内质网-高尔基体交界处的定位。

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如需真实文献,建议使用PubMed或Google Scholar检索关键词“ANKRD27 antibody”或“ANKRD27 function”,并筛选涉及抗体应用的实验研究。

背景信息

The ANKRD27 antibody targets the ANKRD27 protein, also known as VARP or sVAP, encoded by the ANKRD27 gene in humans. This protein contains multiple ankyrin repeat domains and a VPS9 domain, suggesting roles in intracellular trafficking and membrane dynamics. ANKRD27/VARP interacts with RAB32 and RAB38 GTPases, regulating lysosome-related organelles and melanosome biogenesis. It also associates with the Retromer complex, influencing endosome-to-Golgi retrograde transport. Studies link ANKRD27 to neurological conditions, including Parkinson’s disease, through its interaction with LRRK2. a kinase mutated in familial PD. Additionally, it may modulate immune responses by affecting MHC class I trafficking. The ANKRD27 antibody is widely used in research to study protein localization, expression levels in disease models, and molecular pathways involving vesicular transport. Commercial antibodies are validated for techniques like Western blotting, immunofluorescence, and immunohistochemistry. Recent investigations explore its potential as a biomarker or therapeutic target in neurodegenerative disorders and cancer. Ongoing research aims to clarify its precise mechanistic contributions to membrane trafficking and disease pathogenesis.

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