关键信息
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基因名
SARS-CoV-2 NSP16-NSP10
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简介
The multifunctional SARS-CoV-2 PP1ab protein is essential for viral RNA transcription and replication, utilizing proteases for multi-protein cleavage. It inhibits host translation by binding to the 40S subunit and blocks ribosomal mRNA entry channels, thereby hindering the antiviral response. NSP16/NSP10 Protein, Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2) (His) is the recombinant virus-derived NSP16/NSP10, expressed by E. coli, with His labeled tag.
- 应用
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种属
Virus
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表达系统
E. coli
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P0DTD1-1
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表达区间
P0DTD1-1 (S6799-N7096&A4254-Q4392)
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
The study of the SARS-CoV-2 NSP16-NSP10 recombinant protein has gained significant attention due to its critical role in the viral lifecycle. NSP16 is a methyltransferase that modifies the viral RNA cap structure, facilitating its escape from host immune detection and enhancing its translation efficiency. NSP10 serves as a crucial co-factor for NSP16, enhancing its methyltransferase activity. Understanding the interactions between NSP16 and NSP10 is essential for elucidating the molecular mechanisms of SARS-CoV-2 pathogenesis. Additionally, insights into this complex could inform the development of antiviral strategies aimed at disrupting viral replication. Given the ongoing global challenges posed by COVID-19, characterizing recombinant NSP16-NSP10 could lead to novel therapeutic approaches or the design of effective vaccines. The exploration of this protein complex is thus a vital step in the broader effort to combat SARS-CoV-2 and future coronaviruses. By investigating its structure and function, researchers hope to uncover new targets for intervention, ultimately contributing to public health responses against viral outbreaks.
