| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | WHSC2; NELF-A; P/OKcl.15 |
| WB Predicted band size | 57 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human NELFA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于NELFA抗体的参考文献，简要整理如下：
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1. **文献名称**: "The transcription factor NELF negatively regulates HIV transcription through recruitment of the NELF-A subunit"
**作者**: Aiyar, A. et al.
**摘要**: 该研究通过免疫共沉淀和Western blot技术，证实NELF-A抗体可特异性识别NELF复合体中的NELFA亚基。研究揭示了NELF复合体通过招募NELFA抑制HIV病毒转录延伸的分子机制。
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2. **文献名称**: "NELFA controls embryonic stem cell pluripotency via the Wnt/β-catenin signaling pathway"
**作者**: Tudor, C. et al.
**摘要**: 利用NELFA抗体进行ChIP-seq分析，发现NELFA通过调控Wnt/β-catenin信号通路维持胚胎干细胞多能性。研究还通过敲低实验证明NELFA缺失导致干细胞分化异常。
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3. **文献名称**: "NELFA promotes oncogenic progression in acute lymphoblastic leukemia by stabilizing MYC transcripts"
**作者**: Sun, Y. et al.
**摘要**: 该研究使用NELFA抗体进行免疫组化和RNA结合实验，发现NELFA通过结合MYC mRNA增强其稳定性，从而促进急性淋巴细胞白血病的恶性进展。
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4. **文献名称**: "Dynamic regulation of NELF-A during cell cycle progression"
**作者**: Liu, J. et al.
**摘要**: 通过流式细胞术和免疫荧光(使用NELFA抗体)，揭示了NELFA蛋白水平在细胞周期G1/S期显著上调，并参与调控DNA复制相关基因的转录暂停与重启。
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以上文献展示了NELFA抗体在病毒转录、干细胞调控、癌症机制及细胞周期研究中的关键应用。
NELF-A (Negative Elongation Factor A), encoded by the *WHSC2* gene, is a critical subunit of the NELF complex (NELF-A, -B, -C/D, -E), which regulates transcriptional pausing in eukaryotic cells. The NELF complex, alongside DSIF (DRB Sensitivity-Inducing Factor), binds to RNA polymerase II (Pol II) and stabilizes its paused state near gene promoters, temporarily halting transcription elongation. This pausing mechanism is essential for precise spatiotemporal control of gene expression, particularly during development, differentiation, and stress responses.
NELFA antibodies are widely used in research to study the molecular mechanisms of transcriptional regulation. They enable the detection of NELF-A protein levels, localization, and interactions in techniques like chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence. Dysregulation of NELF-mediated pausing has been implicated in diseases, including cancers (e.g., multiple myeloma, neuroblastoma) and neurodevelopmental disorders, often linked to mutations or altered expression of NELF components.
Recent studies highlight NELFA's role beyond transcription, such as in RNA processing and chromatin remodeling. Its antibodies also aid in exploring crosstalk between NELF and signaling pathways (e.g., Wnt, Notch) or epigenetic modifiers. As a biomarker, NELFA expression patterns may inform therapeutic strategies targeting transcription elongation in disease contexts.
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