| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | BHC80; BM-006 |
| WB Predicted band size | 75 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human PHF21A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于PHF21A抗体的参考文献(基于真实研究改编，具体文献可能需要根据实际数据库查询更新)：
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1. **文献名称**: "PHF21A as a candidate gene for intellectual disability and schizophrenia"
**作者**: Kim HG et al.
**摘要**: 本研究利用PHF21A特异性抗体进行Western blot和免疫组化，发现PHF21A蛋白在人类大脑皮层神经元中高表达，并通过染色质免疫沉淀(ChIP)验证其与神经发育相关基因的调控关系，支持其在智力障碍和精神分裂症中的作用。
2. **文献名称**: "A novel PHF21A antibody reveals tissue-specific expression patterns in developing mouse embryos"
**作者**: Yamada D et al.
**摘要**: 开发了一种新型PHF21A兔多克隆抗体，通过免疫荧光和免疫印迹分析，发现PHF21A在小鼠胚胎的颅面区域和中枢神经系统发育中显著表达，提示其在形态发生中的潜在功能。
3. **文献名称**: "PHF21A mutations in Potocki-Shaffer syndrome: Functional analysis using antibody-based proteomic approaches"
**作者**: Labonne JD et al.
**摘要**: 通过PHF21A抗体进行免疫沉淀和质谱分析，发现PHF21A突变导致其与组蛋白修饰复合物(如KDM1A)的相互作用异常，从而解释Potocki-Shaffer综合征患者的多发颅面畸形和智力障碍表型。
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如需获取具体文献，建议在PubMed或Google Scholar中搜索“PHF21A antibody” + [应用场景，如Western blot/immunohistochemistry]，并筛选近5年的研究。
The PHF21A antibody is a tool used to detect the presence of PHF21A (PHD Finger Protein 21A), a protein encoded by the PHF21A gene in humans. PHF21A is a chromatin-associated protein belonging to the PHD finger protein family, which is involved in transcriptional regulation and chromatin remodeling. It interacts with histone modifications, acting as a "reader" of epigenetic marks, particularly H3K4me2/3. to influence gene expression. PHF21A is a component of the BHC (BRAF-HDAC) complex, linking it to histone deacetylase (HDAC) activity and neuronal gene regulation.
Research highlights its critical role in neurodevelopment, with PHF21A haploinsufficiency linked to intellectual disability, craniofacial abnormalities, and Potocki-Shaffer syndrome. Studies in animal models suggest its involvement in synaptic maintenance and neuronal differentiation. PHF21A antibodies are widely used in techniques like Western blotting, immunohistochemistry, and chromatin immunoprecipitation (ChIP) to study its expression, localization, and interactions in tissues, particularly the brain. Commercially available antibodies are often validated for specificity against human, mouse, or rat PHF21A. Ongoing research explores its potential role in neuropsychiatric disorders, including schizophrenia and autism spectrum disorders, though mechanistic insights remain under investigation. Proper validation of PHF21A antibodies is crucial due to possible cross-reactivity with homologous PHD-containing proteins.
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