| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OXSR1 |
| Uniprot No | O95747 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-527aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMSEDSSALPWSINRDDYELQEVIGSGA TAVVQAAYCAPKKEKVAIKRINLEKCQTSMDELLKEIQAMSQCHHPNIVS YYTSFVVKDELWLVMKLLSGGSVLDIIKHIVAKGEHKSGVLDESTIATIL REVLEGLEYLHKNGQIHRDVKAGNILLGEDGSVQIADFGVSAFLATGGDI TRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADIWSFGITAIELATGAAPY HKYPPMKVLMLTLQNDPPSLETGVQDKEMLKKYGKSFRKMISLCLQKDPE KRPTAAELLRHKFFQKAKNKEFLQEKTLQRAPTISERAKKVRRVPGSSGR LHKTEDGGWEWSDDEFDEESEEGKAAISQLRSPRVKESISNSELFPTTDP VGTLLQVPEQISAHLPQPAGQIATQPTQVSLPPTAEPAKTAQALSSGSGS QETKIPISLVLRLRNSKKELNDIRFEFTPGRDTAEGVSQELISAGLVDGR DLVIVAANLQKIVEEPQSNRSVTFKLASGVEGSDIPDDGKLIGFAQLSIS |
| 预测分子量 | 60 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OXSR1重组蛋白的模拟参考文献示例(仅供参考，实际文献需通过学术数据库验证)：
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1. **标题**：*OXSR1 regulates NKCC1 phosphorylation and cellular ion homeostasis under oxidative stress*
**作者**：Tao, X., et al.
**摘要**：研究利用重组OXSR1蛋白，揭示了其在氧化应激条件下通过磷酸化NKCC1(钠钾氯共转运体)调控细胞离子平衡的分子机制，证实OXSR1对细胞容积调节的关键作用。
2. **标题**：*Structural and functional characterization of the OXSR1 kinase domain*
**作者**：Smith, J.R., et al.
**摘要**：通过重组OXSR1激酶结构域蛋白的结晶和活性分析，解析了其三维结构，并发现其ATP结合口袋的独特构象，为靶向OXSR1的药物设计提供结构基础。
3. **标题**：*OXSR1 modulates inflammatory responses via MAPK signaling in macrophages*
**作者**：Kim, Y., & Lee, S.
**摘要**：利用重组OXSR1蛋白进行体外激酶实验，证明其通过激活p38 MAPK通路增强巨噬细胞炎症反应，提示OXSR1在免疫调控中的潜在作用。
4. **标题**：*OXSR1-mediated metabolic adaptation in breast cancer cells*
**作者**：Chen, L., et al.
**摘要**：研究发现乳腺癌细胞中OXSR1重组蛋白可增强AMPK信号通路活性，促进能量代谢重编程，从而支持肿瘤细胞在缺氧环境中的存活。
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**注意**：以上文献信息为示例性质，实际研究中请通过PubMed、Web of Science或Google Scholar等平台检索真实发表的论文。
OXSR1 (Oxidative Stress Responsive 1) is a serine/threonine kinase belonging to the STE20 family, primarily involved in cellular stress response and ion homeostasis regulation. It acts downstream of the WNK (With No Lysine) kinase pathway, phosphorylating key effector proteins like SPAK (STE20/SPS1-related proline-alanine-rich kinase) to modulate ion transporters, including NKCC1 (Na⁺-K⁺-2Cl⁻ cotransporter) and NCC (Na⁺-Cl⁻ cotransporter). This interaction plays a critical role in maintaining osmotic balance, cell volume, and blood pressure regulation. Structurally, OXSR1 contains an N-terminal catalytic domain and a C-terminal regulatory domain that facilitates binding to scaffolding proteins or stress-activated signaling complexes.
Recombinant OXSR1 protein is engineered for in vitro studies to dissect its kinase activity, substrate specificity, and regulatory mechanisms. Produced via bacterial or mammalian expression systems, it often includes tags (e.g., GST, His-tag) for purification and detection. Researchers use it to map phosphorylation sites, analyze interactions with WNK-SPAK complexes, and screen kinase inhibitors. Its role in oxidative stress pathways—particularly in response to reactive oxygen species (ROS) or hypertonic conditions—has linked OXSR1 to diseases like hypertension, neurodegenerative disorders, and cancer metastasis. Recombinant OXSR1 also aids in structural studies (e.g., X-ray crystallography) to resolve active conformations and design targeted therapies. Recent studies highlight its potential as a biomarker for drug resistance in chemotherapy, emphasizing its dual function as a stress sensor and signaling hub. However, its pleiotropic effects across tissues necessitate context-specific analysis using recombinant tools to avoid off-target implications in therapeutic development.
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