| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | P4HB |
| Uniprot No | P07237 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-508aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MDAPEEEDHV LVLRKSNFAE ALAAHKYLLV EFYAPWCGHC KALAPEYAKA AGKLKAEGSE IRLAKVDATE ESDLAQQYGV RGYPTIKFFR NGDTASPKEY TAGREADDIV NWLKKRTGPA ATTLPDGAAA ESLVESSEVA VIGFFKDVES DSAKQFLQAA EAIDDIPFGI TSNSDVFSKY QLDKDGVVLF KKFDEGRNNF EGEVTKENLL DFIKHNQLPL VIEFTEQTAP KIFGGEIKTH ILLFLPKSVS DYDGKLSNFK TAAESFKGKI LFIFIDSDHT DNQRILEFFG LKKEECPAVR LITLEEEMTK YKPESEELTA ERITEFCHRF LEGKIKPHLM SQELPEDWDK QPVKVLVGKN FEDVAFDEKK NVFVEFYAPW CGHCKQLAPI WDKLGETYKD HENIVIAKMD STANEVEAVK VHSFPTLKFF PASADRTVID YNGERTLDGF KKFLESGGQD GAGDDDDLED LEEAEEPDME EDDDQKAVKD EL |
| 预测分子量 | 57 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于P4HB重组蛋白的3篇参考文献示例(注：文献信息为假设性概括，实际引用需以真实文献为准)：
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1. **文献名称**：*Recombinant Human P4HB Protein Enhances Collagen Deposition in Wound Healing Models*
**作者**：Smith A, et al.
**摘要**：研究报道了重组人P4HB蛋白通过促进胶原蛋白羟基化修饰，加速小鼠皮肤创伤模型中细胞外基质的重塑，证明其在组织修复中的潜在应用价值。
2. **文献名称**：*Expression and Purification of P4HB in E. coli for Structural Analysis*
**作者**：Chen L, et al.
**摘要**：本文描述了一种利用大肠杆菌高效表达重组P4HB蛋白的方法，并通过X射线晶体学解析其三维结构，揭示了其与胶原前体结合的关键位点。
3. **文献名称**：*P4HB Overexpression in Cancer: Implications for Tumor Metastasis*
**作者**：Wang Y, et al.
**摘要**：研究发现P4HB在多种肿瘤细胞中高表达，其重组蛋白可激活ERK信号通路，促进癌细胞侵袭转移，提示其作为癌症治疗靶点的可能性。
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(如需真实文献，建议通过PubMed或Web of Science检索关键词“P4HB recombinant protein”或“Prolyl 4-hydroxylase beta subunit”。)
P4HB (Prolyl 4-Hydroxylase Subunit Beta), also known as Protein Disulfide Isomerase A1 (PDIA1), is a multifunctional enzyme encoded by the P4HB gene. It plays a critical role in post-translational modification of collagen, serving as the beta subunit of prolyl 4-hydroxylase (P4H), an enzyme essential for stabilizing collagen triple-helix structures by hydroxylating proline residues. This hydroxylation is vital for collagen’s thermal stability and mechanical strength, impacting connective tissue integrity. Beyond collagen synthesis, P4HB acts as a protein disulfide isomerase (PDI), catalyzing disulfide bond formation, rearrangement, and reduction during protein folding in the endoplasmic reticulum. This dual functionality links P4HB to cellular stress responses, quality control of secreted proteins, and extracellular matrix homeostasis.
Recombinant P4HB protein is engineered for research and therapeutic applications. Its production typically involves heterologous expression systems (e.g., E. coli, mammalian cells) to study collagen-related disorders, such as osteogenesis imperfecta or fibrosis, where aberrant collagen hydroxylation contributes to pathology. In drug development, recombinant P4HB is explored for modulating collagen metabolism in fibrotic diseases (e.g., liver cirrhosis) or enhancing wound healing. Additionally, P4HB’s PDIA1 activity is implicated in cancer progression, thrombosis, and neurodegenerative diseases, making it a potential therapeutic target. For instance, inhibitors of P4HB’s PDI activity are investigated for antitumor or antithrombotic effects. Recombinant variants may also serve as tools to dissect molecular mechanisms of protein folding diseases, such as Alzheimer’s, where misfolded proteins accumulate. Overall, P4HB recombinant protein bridges fundamental research and clinical innovation in collagen biology and ER stress-related pathologies.
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