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产品描述：人钠钾转运ATP酶亚基α-1(ATP1A1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ATP1A1含量。ATP1A1 是一种重要靶点，它编码钠钾-ATP 酶的α1亚基，在维持细胞内外离子平衡等生理过程中意义重大。研究发现其异常表达或功能改变与多种疾病相关，如肿瘤、神经系统疾病等，目前致力于探索靶向它的作用机制以开发新的治疗策略。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，产品适用于心血管疾病模型、神经退行性疾病机制、药物靶点筛选等基础研究领域，尤其可为离子通道相关蛋白的功能研究、病理模型构建及化合物干预效果评价提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：A1A1 ELISA Kit; AT1A1 ELISA Kit; AT1A1_HUMAN ELISA Kit; ATP1A1 ELISA Kit; Atpa-1 ELISA Kit; ATPase Na+/K+ transporting alpha 1 polypeptide ELISA Kit; ATPase Na+/K+ transporting subunit alpha 1 ELISA Kit; BC010319 ELISA Kit; EC 3.6.3.9 ELISA Kit; MGC3285 ELISA Kit; MGC38419 ELISA Kit; MGC51750 ELISA Kit; Na K ATPase alpha A catalytic polypeptide ELISA Kit; Na K ATPase catalytic subunit alpha A protein ELISA Kit; Na(+)/K(+) ATPase 1 ELISA Kit; Na(+)/K(+) ATPase alpha-1 subunit ELISA Kit; Na+; K+ ATPase alpha subunit ELISA Kit; Na+/K+ ATPase alpha 1 subunit ELISA Kit; Na+/K+ ATPase 1 ELISA Kit; Na,K ATPase alpha 1 subunit ELISA Kit; Nkaa1b ELISA Kit; Sodium potassium ATPase alpha 1 polypeptide ELISA Kit; Sodium pump 1 ELISA Kit; Sodium pump subunit alpha-1 ELISA Kit; sodium-potassium ATPase catalytic subunit alpha-1 ELISA Kit; Sodium/potassium-transporting ATPase subunit alpha-1 ELISA Kit
缩写：ATP1A1
Uniprot No.：P05023
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ATP1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 80-100 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 85-104 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 92-104 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 82-110 | |||||
回收率：
| The recovery of human ATP1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 84-106 | ||||
| EDTA plasma (n=4) | 98 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.6852.6972.6912.5345001.9541.9651.9601.8032501.1041.1251.1150.9581250.7010.7320.7170.56062.50.4460.4400.4430.28631.20.3260.3150.3210.16415.60.2670.2550.2610.10400.1530.1610.157 | ||||||
货期：3-5 working days
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