首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠β淀粉样蛋白1-42(Aβ1-42)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Aβ1-42含量。Aβ1-42是重要的阿尔茨海默病相关靶点。在患者脑内该蛋白异常聚集形成淀粉样斑块，破坏神经细胞功能与结构。研究聚焦其产生、聚集过程及与神经毒性的关联，旨在通过抑制生成、促进清除等机制来改善病情、研发治疗药物。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于阿尔茨海默病小鼠模型构建、Aβ病理机制研究、药物干预效果评估等实验场景，为神经科学领域提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Amyloid beta 42(ABeta 42),amyloid beta peptide 1-42,Aβ1-42,amyloid βpeptide 1-42
缩写：Aβ1-42
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Aβ1-42 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-99 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 85-105 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 90-105 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-100 | |||||
回收率：
| The recovery of mouse Aβ1-42 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 93 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.4902.4512.4712.337 5002.0491.9852.0171.883 2501.3571.3851.3711.237 1250.8660.8370.8520.718 62.50.5170.5240.5210.387 31.20.3470.3550.3510.217 15.60.2440.2280.2360.102 00.1350.1320.134 | ||||||
货期：3-5 working days
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