| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DECRD; MNADK; NADKD1; C5orf33 |
| WB Predicted band size | 49 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Fusion protein of human NADK2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4条关于NADK2抗体的虚构参考文献示例(仅供模拟参考，非真实文献)：
1. **"Mitochondrial NADK2 regulates redox homeostasis via NADPH generation"**
*Author: Li, X. et al. (2022)*
摘要：研究利用特异性NADK2抗体，通过Western blot和免疫荧光验证其在哺乳动物细胞线粒体中的定位，发现NADK2通过调控NADPH水平影响氧化应激反应。
2. **"NADK2 expression correlates with cancer cell metabolic reprogramming"**
*Author: Gonzalez, R. & Park, J. (2021)*
摘要：通过免疫组化分析NADK2抗体在肿瘤组织中的表达，发现其高表达与肝癌细胞脂质合成增强相关，提示其可能作为代谢治疗靶点。
3. **"A novel NADK2 antibody reveals its role in ferroptosis resistance"**
*Author: Chen, L. et al. (2020)*
摘要：开发高特异性NADK2单克隆抗体，证实其通过维持线粒体NADPH抑制铁死亡，为神经退行性疾病研究提供工具。
4. **"CRISPR screening identifies NADK2 as a modulator of mitochondrial apoptosis"**
*Author: Müller, S. et al. (2019)*
摘要：结合CRISPR基因编辑和NADK2抗体验证，发现NADK2缺失导致Bcl-2家族蛋白活性改变，影响细胞凋亡通路。
(注：以上为模拟生成内容，实际文献需通过PubMed/Google Scholar等平台检索确认。)
The NADK2 antibody is a research tool designed to detect and study NAD kinase 2 (NADK2), a mitochondrial enzyme critical for cellular metabolism. NADK2 catalyzes the phosphorylation of nicotinamide adenine dinucleotide (NAD⁺) to generate NADP⁺, a coenzyme essential for redox balance, antioxidant defense (via glutathione regeneration), and biosynthetic pathways like lipid synthesis. Unlike its cytosolic counterpart NADK1. NADK2 operates within mitochondria, linking its activity to energy metabolism and mitochondrial function.
NADK2 antibodies are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to investigate protein expression, localization, and regulation in various tissues and disease models. Researchers employ these antibodies to explore NADK2's role in metabolic disorders, cancer (where altered NADPH levels affect tumor growth), and neurodegenerative diseases. Dysregulation of NADK2 has been implicated in mitochondrial dysfunction and oxidative stress, making it a potential therapeutic target.
The specificity of NADK2 antibodies is validated using knockout cell lines or tissues to ensure minimal cross-reactivity with other NAD kinases. Commercial antibodies often target conserved regions of the protein across species like humans, mice, and rats. Recent studies also associate NADK2 mutations with rare genetic disorders, such as hyperphosphatasia or metabolic syndromes, highlighting its physiological importance. By enabling precise detection, NADK2 antibodies contribute to advancing our understanding of mitochondrial metabolism and its implications in health and disease.
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