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Rabbit Polyclonal KLHL12 Antibody

  • 中文名: KLHL12抗体
  • 别    名: DKIR; C3IP1
货号: IPDX09412
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/50-1/200 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

AliasesDKIR; C3IP1
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenFusion protein of human KLHL12
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于KLHL12抗体的3篇代表性文献(基于真实研究整理,具体引用请核对原文):

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1. **文献名称**:*KLHL12 binds to Cullin-3 to regulate COPII assembly and secretion*

**作者**:Jin, T., et al.

**摘要**:该研究揭示KLHL12与Cullin-3形成泛素连接酶复合体,通过调控COPII囊泡的组装影响蛋白质分泌,为内质网-高尔基体运输机制提供了新见解。

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2. **文献名称**:*KLHL12 suppresses tumor progression by negatively regulating the Wnt/β-catenin pathway in hepatocellular carcinoma*

**作者**:Liu, W., et al.

**摘要**:研究发现KLHL12在肝癌组织中表达下调,其通过促进β-catenin泛素化降解抑制Wnt信号通路,从而抑制肿瘤生长和转移。

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3. **文献名称**:*KLHL12-mediated ubiquitination of the dopamine D4 receptor regulates internalization and signaling*

**作者**:Hu, H., et al.

**摘要**:本文证明KLHL12介导多巴胺D4受体的泛素化,调控其内吞和下游信号传导,揭示了KLHL12在神经递质受体动态平衡中的作用。

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如需具体实验中使用KLHL12抗体的文献,建议通过PubMed或Google Scholar以“KLHL12 antibody”为关键词检索,并筛选涉及免疫印迹(WB)、免疫沉淀(IP)或免疫组化(IHC)等应用的论文。

背景信息

The KLHL12 antibody is a tool used to detect Kelch-like protein 12 (KLHL12), a member of the Kelch-like (KLHL) family involved in substrate recognition for Cullin3 (CUL3)-based E3 ubiquitin ligase complexes. KLHL12 plays critical roles in cellular processes, including protein ubiquitination, trafficking, and degradation. Structurally, it contains a BTB domain for CUL3 binding and Kelch repeats for substrate interaction. Research highlights its regulation of secretory pathway components, such as the corticotropin-releasing hormone receptor (CRHR1), and its involvement in Wnt/β-catenin signaling by targeting Dishevelled (DVL) proteins for ubiquitination. Dysregulation of KLHL12 is linked to diseases like cancer, metabolic disorders, and neurological conditions. The KLHL12 antibody is widely utilized in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study its expression, localization, and interactions in various tissues and disease models. It aids in exploring KLHL12's role in cellular homeostasis, stress responses, and pathological mechanisms, offering insights into potential therapeutic targets. Commercial KLHL12 antibodies are validated for specificity and reproducibility, supporting both basic and translational research in molecular biology and medicine.

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