首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：绵羊加压素-神经垂体素运载蛋白-2-和肽素(AVP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AVP含量。AVP即精氨酸加压素，是一种具有多种生理功能的神经肽。其参与调节水盐平衡、血压稳定等。在研究机制上，它通过与特定受体结合，调控细胞内信号传导通路，影响肾脏、心血管等多种组织器官功能，相关研究为治疗相关疾病提供了方向。试剂盒检测范围为1.56 pg/mL-100 pg/mL，适用于科研领域探究绵羊心血管系统、肾脏功能或应激生理机制的相关研究，特别是在动物模型的水代谢异常、抗利尿激素相关调控机制等方向具有重要应用价值，为神经内分泌领域的分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ADH, ARVP, AVP-NPII, AVRP, VP, antidiuretic hormone|arginine vasopressin-neurophysin II|neurohypophyseal|vasopressin-neurophysin II-copeptin
缩写：AVP
种属：Ovis aries (Sheep)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep AVP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-94 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 95-105 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-95 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of sheep AVP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 92 | 86-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.5002.3452.4232.296501.9221.8891.9061.779251.2241.2531.2391.11212.50.7550.7760.7660.6396.250.4450.4640.4550.3283.120.3160.3030.3100.1831.560.2160.2110.2140.08700.1280.1260.127 | ||||||
货期：3-5 working days
×
