| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | sen15; C1orf19 |
| WB Predicted band size | 19 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于TSEN15抗体的3篇参考文献示例(注：以下为假设性文献，实际文献需通过数据库验证)：
---
1. **文献名称**：*"TSEN15 mutations disrupt tRNA splicing endonuclease complex integrity in pontocerebellar hypoplasia"*
**作者**：Smith A, et al.
**摘要**：本研究探讨了TSEN复合体亚基(包括TSEN15)在PCH患者中的突变影响。通过TSEN15抗体进行Western blot和免疫共沉淀实验，发现特定突变导致复合体稳定性下降，影响tRNA剪接功能，揭示了TSEN15在神经发育中的关键作用。
2. **文献名称**：*"Functional characterization of TSEN15 in zebrafish models of neurodevelopmental disorders"*
**作者**：Zhang L, et al.
**摘要**：利用斑马鱼模型研究TSEN15缺失对神经发育的影响。通过TSEN15抗体检测蛋白表达水平，发现敲低TSEN15导致小脑发育异常，并伴随tRNA加工缺陷，提示其与人类PCH病理机制的相关性。
3. **文献名称**：*"Assembly and dynamics of the human tRNA splicing endonuclease complex"*
**作者**：Braun S, et al.
**摘要**：通过质谱分析和TSEN15抗体的免疫沉淀实验，解析TSEN复合体(TSEN15、TSEN2、TSEN34、TSEN54)的组装机制，证实TSEN15与其他亚基的直接互作对酶活性和底物识别至关重要。
---
如需具体文献，建议在PubMed或Google Scholar中搜索关键词“TSEN15 antibody”、“TSEN complex”或“pontocerebellar hypoplasia TSEN15”获取最新研究。
TSEN15 (tRNA splicing endonuclease subunit 15) is a critical component of the tRNA splicing endonuclease complex, which is essential for the maturation of precursor tRNAs (pre-tRNAs) in eukaryotic cells. This complex, composed of catalytic subunits TSEN2. TSEN34. TSEN54. and the non-catalytic TSEN15. recognizes and cleaves intronic sequences from pre-tRNAs during their processing. TSEN15 plays a structural role in stabilizing the complex and facilitating its interaction with the CLP1 kinase, which is involved in tRNA splicing and RNA metabolism.
Mutations in TSEN15 are linked to pontocerebellar hypoplasia type 2B (PCH2B), a severe neurodevelopmental disorder characterized by progressive brain atrophy, microcephaly, and motor deficits. Research on TSEN15 antibodies focuses on elucidating the molecular mechanisms underlying tRNA splicing defects and their contribution to neurodegenerative pathologies. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect TSEN15 expression, study its localization, and assess interactions within the tRNA splicing machinery.
TSEN15 antibodies also aid in diagnosing PCH-related disorders and in preclinical studies exploring therapeutic interventions. Their development underscores the importance of understanding tRNA processing in both basic cellular biology and disease contexts.
×
