首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠抗利尿激素/血管加压素/精氨酸加压素(ADH/VP/AVP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的AVP含量。AVP即精氨酸加压素，它在人体生理中作用关键，可调节水盐平衡、影响血管张力等。在研究机制方面，科研聚焦其受体亚型，探索通过作用于这些受体调控相关生理过程，有望为治疗尿崩症、心血管疾病等提供新策略。试剂盒检测范围为1.25 pg/mL-80 pg/mL，常用于内分泌机制、水盐代谢研究及高血压或尿崩症动物模型的科研分析；适用于实验室对神经内分泌调节机制、病理生理模型中的激素动态变化以及药物干预效应等科研场景的定量研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ADH, ARVP, AVP-NPII, AVRP, VP, antidiuretic hormone|arginine vasopressin-neurophysin II|neurohypophyseal|vasopressin-neurophysin II-copeptin
缩写：AVP
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates
检测范围：1.25 pg/mL-80 pg/mL
灵敏度：0.31 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADH/VP/AVP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:4 | Average % | 87 | ||||
| Range % | 82-96 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 90-104 | |||||
回收率：
| The recovery of mouse ADH/VP/AVP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 83-94 | ||||
| EDTA plasma (n=4) | 102 | 98-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected802.5432.5782.5612.373401.8201.8461.8331.645201.1721.1981.1850.997100.7820.7950.7890.60150.4470.4680.4580.2702.50.3230.3390.3310.1431.250.2910.2700.2810.09300.1870.1890.188 | ||||||
货期：3-5 working days
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