首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠β淀粉样蛋白1-40(Aβ1-40)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Aβ1-40含量。Aβ1-40是一种淀粉样蛋白，是阿尔茨海默病（AD）病理过程中的关键分子。它是淀粉样蛋白前体蛋白（APP）的代谢产物，通过β-折叠结构聚集形成老年斑，对神经细胞产生毒性，导致神经元损伤和认知功能下降。Aβ1-40的研究有助于揭示AD的发病机制，并为开发新的治疗策略提供靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，主要应用于神经科学领域的基础研究，包括阿尔茨海默病小鼠模型建立、药物干预后Aβ代谢通路评估、神经毒性作用机制探索等方向，为病理机制解析和潜在治疗策略开发提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：amyloid beta peptide 1-40,Aβ1-40,amyloid β peptide 1-40
缩写：Aβ1-40
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Aβ1-40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 80-97 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 83-100 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 83-98 | |||||
回收率：
| The recovery of mouse Aβ1-40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 93-106 | ||||
| EDTA plasma (n=4) | 89 | 86-92 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.6372.7392.6882.51210002.1152.0922.1041.9285001.3181.2871.3031.1272500.8240.8100.8170.6411250.4910.5040.4980.32262.50.3220.3150.3190.14331.250.2650.2730.2690.09300.1720.1800.176 | ||||||
货期：3-5 working days
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