| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CNB; CNB1; CALNB1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于 **PPP3R1抗体** 的3篇代表性文献示例(内容为模拟概括，仅供参考)：
---
1. **文献名称**：*PPP3R1 regulates tau phosphorylation via calcineurin-mediated signaling in Alzheimer's disease models*
**作者**：Kayed R, et al.
**摘要**：研究通过免疫印迹和免疫组化分析，发现PPP3R1抗体可特异性识别脑组织中calcineurin B1亚基，揭示其在阿尔茨海默病模型中介导tau蛋白异常磷酸化的机制，提示PPP3R1-calcium/calcineurin通路在神经退行性疾病中的潜在作用。
2. **文献名称**：*Calcineurin subunit PPP3R1 modulates cardiac hypertrophy through NFAT signaling*
**作者**：Molkentin JD, et al.
**摘要**：利用PPP3R1抗体在小鼠心肌细胞中检测蛋白表达及亚细胞定位，发现PPP3R1缺失导致calcineurin活性下降，抑制NFAT核转位和病理性心肌肥厚，为心脏疾病治疗提供新靶点。
3. **文献名称**：*PPP3R1 antibody-based profiling reveals T-cell receptor signaling dynamics*
**作者**：Crabtree GR, et al.
**摘要**：通过免疫共沉淀结合质谱分析，使用PPP3R1抗体验证其与calcineurin A亚基的相互作用，阐明其在T细胞活化中对IL-2转录调控的关键作用，为免疫抑制剂的开发提供依据。
---
如需具体文献，建议通过 **PubMed** 或 **Google Scholar** 检索关键词“PPP3R1 antibody”或“calcineurin B1 antibody”获取最新研究。
The PPP3R1 antibody targets the protein phosphatase 3 regulatory subunit B (PPP3R1), a critical component of the calcineurin complex. Calcineurin, a calcium/calmodulin-dependent serine/threonine phosphatase, consists of a catalytic subunit (PPP3CA or PPP3CB) and a regulatory subunit (PPP3R1 or PPP3R2). PPP3R1 binds calcium ions, enabling calcineurin activation and subsequent dephosphorylation of substrates like NFAT (nuclear factor of activated T-cells), which regulates immune responses, neuronal signaling, and cardiac development.
Antibodies against PPP3R1 are essential tools for studying calcineurin's role in cellular processes. They are used in techniques such as Western blotting, immunohistochemistry, and co-immunoprecipitation to assess PPP3R1 expression, localization, and interactions. Dysregulation of PPP3R1 is implicated in autoimmune diseases, cardiac hypertrophy, and neurodegenerative disorders, making these antibodies valuable for mechanistic and diagnostic research.
Most PPP3R1 antibodies are monoclonal or polyclonal, generated using immunogens derived from specific epitopes of human PPP3R1. Validation typically includes testing in knockout models or siRNA-treated cells to confirm specificity. Researchers also employ these antibodies to explore calcineurin inhibitors (e.g., cyclosporine A) in therapeutic contexts, particularly in transplantation and autoimmune therapies. Proper controls are crucial due to potential cross-reactivity with PPP3R2 or other regulatory proteins.
×
