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产品描述：人β-1,4-半乳糖基转移酶1(B4GALT1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、细胞裂解物、组织匀浆样本中的B4GALT1含量。B4GALT1是一种β-1,4-半乳糖基转移酶，参与N-糖基化过程，与多种疾病相关。研究发现，B4GALT1在降低LDL-C和纤维蛋白原水平方面具有重要作用，同时，其在肺腺癌免疫逃逸中发挥关键作用，可能成为治疗靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于基础科研中探索B4GALT1在肿瘤微环境、代谢调控或细胞分化等领域的生物学功能，亦可配合细胞实验或动物模型进行蛋白表达动态分析。为生命科学领域的机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：B4GALT1; GGTB2; Beta-1,4-galactosyltransferase 1; Beta-1,4-GalTase 1; Beta4Gal-T1; b4Gal-T1; Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase; Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase; Lactose synthase A protein; N-acetyllactosamine synthase; Nal synthase; Neolactotriaosylceramide beta-1,4-galactosyltransferase; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1
缩写：B4GALT1
Uniprot No.：P15291
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell lysates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human B4GALT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 96 | |||||
| Range % | 90-102 | ||||||
| 1:2 | Average % | 89 | |||||
| Range % | 84-95 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 87-99 | ||||||
| 1:8 | Average % | 92 | |||||
| Range % | 88-96 | ||||||
回收率：
| The recovery of human B4GALT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 86 | 80-90 | |||||
| EDTA plasma (n=4) | 100 | 95-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.2012.1532.1772.02251.7311.6591.6951.5402.51.2911.2641.2781.1231.250.8360.8920.8640.7090.6250.6470.6700.6590.5040.3120.3930.3750.3840.2290.1560.2640.2710.2680.11300.1530.1560.155 | |||||||
货期：3-5 working days
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