关键信息
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基因名
CDK5
- 应用
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别名
Cell division protein kinase 5Serine/threonine-protein kinase PSSALRETau protein kinase II catalytic subunit ;TPKII catalytic subunit
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种属
Human
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表达系统
E. coli
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标签
N- His-SUMO
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q00535
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表达区间
1-292aa
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分子量
49.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
Cyclin-dependent kinase 5 (CDK5) is a serine/threonine kinase that plays a crucial role in the regulation of neuronal development and function. Unlike other cyclin-dependent kinases that are primarily involved in cell cycle regulation, CDK5 is activated by association with non-cyclin proteins, particularly p35 and p39, which are neuron-specific activators. Dysregulation of CDK5 activity has been implicated in various neurodegenerative diseases, including Alzheimer's disease, Parkinson's disease, and epilepsy, highlighting its importance in maintaining neuronal health. Research has documented that aberrant CDK5 activity, often resulting from the cleavage of its activators, leads to hyperphosphorylation of tau protein and subsequent neurofibrillary tangles—a hallmark of Alzheimer's pathology. Furthermore, CDK5 is involved in multiple signaling pathways that affect neuronal cell functions, such as synaptic plasticity, neuronal migration, and apoptosis. Therefore, understanding the regulatory mechanisms of CDK5 and its interactions with other molecular players is vital for developing therapeutic strategies aimed at alleviating the consequences of its dysregulation. Current studies focus on elucidating the structure-function relationships of CDK5, identifying potential small-molecule inhibitors, and exploring its role in synaptic function and neuronal survival, which may pave the way for innovative treatments targeting CDK5-related disorders.
