Mouse β2-microglobulin,BMG/β2-MG ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E13053m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠β2微球蛋白(BMG/β2-MG)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆样本中的B2M含量。B2M即β2-微球蛋白，是MHCⅠ类分子的轻链，广泛存在于有核细胞表面。其在免疫识别、抗原呈递等方面发挥重要作用。研究中，它与多种疾病如肿瘤、自身免疫病相关，通过检测其水平变化等机制，助力疾病的诊断、病情监测及预后评估。试剂盒检测范围为0.156 μg/ml-10 μg/ml.，适用于多种科研场景，包括但不限于肾脏疾病模型中肾小管功能评估、免疫系统相关基因敲除动物的β2 - MG动态监测、肿瘤微环境中免疫调控机制研究以及尿液生物标志物探索等领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
B2mBeta-2-microglobulin ELISA kit
缩写：
B2M
Uniprot No.：
P01887
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, urine, tissue homogenates
检测范围：
0.156 μg/ml-10 μg/ml.
灵敏度：
0.156 μg/ml.
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse BMG/β2-MG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	88
	Range %	83-93
1:2	Average %	92
	Range %	85-98
1:4	Average %	86
	Range %	82-91
1:8	Average %	93
	Range %	85-99

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


μg/ml	OD1	OD2	Average
10	0.219	0.230	0.224
5	0.305	0.317	0.311
2.5	0.445	0.428	0.436
1.25	0.538	0.572	0.555
0.625	0.815	0.821	0.818
0.312	1.099	1.053	1.076
0.156	1.503	1.484	1.494
0	2.235	2.125	2.180