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产品描述：犬β2微球蛋白(BMG/β2-MG)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的B2M含量。B2M全称为β2微球蛋白，其在免疫识别、抗原呈递等过程中发挥重要作用。它与主要组织相容性复合体Ⅰ类分子结合，参与机体免疫监视。研究发现其表达异常与肿瘤、自身免疫病等多种疾病相关，目前研究聚焦于其在疾病发生发展机制及作为治疗靶点的潜力。试剂盒检测范围为0.78 μg/mL-50 μg/mL，适用于科研场景下犬类肾脏功能评估、免疫相关疾病机制研究或药物毒性实验中生物标志物的检测，为动物实验提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：B2M ELISA Kit; Beta-2-microglobulin ELISA Kit; Fragment ELISA Kit
缩写：B2M
Uniprot No.：P19341
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 μg/mL-50 μg/mL
灵敏度：0.2 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog β2-MG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 90 | |||||
| Range % | 83-99 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 85-100 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 85-105 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 88-102 | ||||||
回收率：
| The recovery of dog β2-MG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 86-99 | |||||
| EDTA plasma (n=4) | 95 | 91-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| μg/mlOD1OD2AverageCorrected502.5542.5692.5622.430252.2722.2562.2642.13212.51.7921.8031.7981.6666.251.1531.1801.1671.0353.130.6250.6160.6210.4891.560.3630.3790.3710.2390.780.2610.2640.2630.13100.1350.1290.132 | |||||||
货期：3-5 working days
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