| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PAX8 |
| Uniprot No | Q06710 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-450aa |
| 氨基酸序列 | MPHNSIRSGHGGLNQLGGAFVNGRPLPEVVRQRIVDLAHQGVRPCDISRQLRVSHGCVSKILGRYYETGSIRPGVIGGSKPKVATPKVVEKIGDYKRQNPTMFAWEIRDRLLAEGVCDNDTVPSVSSINRIIRTKVQQPFNLPMDSCVATKSLSPGHTLIPSSAVTPPESPQSDSLGSTYSINGLLGIAQPGSDKRKMDDSDQDSCRLSIDSQSSSSGPRKHLRTDAFSQHHLEPLECPFERQHYPEAYASPSHTKGEQGLYPLPLLNSTLDDGKATLTPSNTPLGRNLSTHQTYPVVADPHSPFAIKQETPEVSSSSSTPSSLSSSAFLDLQQVGSGVPPFNAFPHAASVYGQFTGQALLSGREMVGPTLPGYPPHIPTSGQGSYASSAIAGMVAGSEYSGNAYGHTPYSSYSEAWRFPNSSLLSSPYYYSSTSRPSAPPTTATAFDHL |
| 预测分子量 | 55.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于PAX8重组蛋白的参考文献及其摘要内容概括：
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1. **文献名称**: *"Functional characterization of PAX8 recombinant protein in thyroid cell differentiation"*
**作者**: Kimura S, et al.
**摘要**: 研究利用重组PAX8蛋白探究其在甲状腺滤泡细胞分化中的作用，证实其通过结合特定DNA序列激活甲状腺球蛋白(TG)和甲状腺过氧化物酶(TPO)基因表达，为甲状腺发育机制提供分子基础。
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2. **文献名称**: *"PAX8 recombinant protein purification and its interaction with chromatin modifiers"*
**作者**: Di Palma T, et al.
**摘要**: 报道PAX8重组蛋白在大肠杆菌中的高效表达及纯化方法，并发现其与组蛋白乙酰转移酶p300的相互作用，提示PAX8通过表观遗传调控影响靶基因转录，尤其在甲状腺癌中可能发挥作用。
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3. **文献名称**: *"Development of a PAX8-specific monoclonal antibody using recombinant PAX8 protein for diagnostic applications"*
**作者**: Nonaka D, et al.
**摘要**: 利用重组PAX8蛋白制备高特异性单克隆抗体，验证其在卵巢癌和甲状腺癌组织中的诊断价值，证明其作为病理标志物在区分肿瘤亚型中的可靠性。
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4. **文献名称**: *"Structural analysis of PAX8 DNA-binding domain by recombinant protein crystallography"*
**作者**: Kozmik Z, et al.
**摘要**: 解析重组PAX8蛋白DNA结合域(DBD)的晶体结构，揭示其与靶DNA结合的分子机制，为设计干预PAX8异常活性的小分子药物提供结构基础。
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以上文献涵盖PAX8重组蛋白的功能研究、结构分析、相互作用及临床应用，可作为相关领域研究的参考。
PAX8 (Paired Box 8) is a transcription factor encoded by the *PAX8* gene, belonging to the PAX family of proteins characterized by a conserved paired-box DNA-binding domain. It plays critical roles in embryonic development, particularly in the formation of the thyroid gland, kidneys, and Müllerian duct-derived tissues. PAX8 regulates organ-specific gene expression by binding to target DNA sequences, thereby influencing cell differentiation and proliferation. Its expression is tightly tissue-restricted, with prominence in thyroid follicular cells, renal tubular cells, and epithelial cells of the female reproductive tract.
Recombinant PAX8 protein is engineered through molecular cloning techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. The protein is purified using affinity tags (e.g., His-tag) and validated via Western blot, ELISA, or functional assays. Recombinant PAX8 serves as a vital tool for studying thyroid development, renal pathophysiology, and reproductive system disorders. It is widely used in *in vitro* studies to dissect signaling pathways, gene regulation mechanisms, and interactions with co-factors like NKX2-1 or WT1.
Clinically, PAX8 has emerged as a biomarker for diagnosing carcinomas of thyroid, ovarian, and renal origins. Recombinant PAX8 antibodies aid in immunohistochemical staining to distinguish tumor types. Additionally, its role in maintaining cellular identity makes it a candidate for regenerative medicine and cancer therapy research. Dysregulation of PAX8 is linked to congenital hypothyroidism, kidney malformations, and cancers, underscoring its therapeutic and diagnostic relevance. Advances in recombinant technology continue to enhance its accessibility for both basic research and clinical applications.
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