| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTH |
| Uniprot No | P01270 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 32-115aa |
| 氨基酸序列 | SVSEIQLMHN LGKHLNSMER VEWLRKKLQD VHNFVALGAP LAPRDAGSQR PRKKEDNVLV ESHEKSLGEA DKADVNVLTK AKSQ |
| 预测分子量 | 9.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PTH(甲状旁腺激素)重组蛋白的参考文献摘要概括，供参考：
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1. **文献名称**：*Expression and Purification of Recombinant Human Parathyroid Hormone (rhPTH) in Escherichia coli*
**作者**：Huang Y, et al. (2015)
**摘要**：研究报道了一种高效利用大肠杆菌表达系统生产重组人PTH的方法，通过优化密码子和纯化步骤，获得了高纯度的活性蛋白，并验证了其促进骨细胞增殖的生物活性。
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2. **文献名称**：*Pharmacodynamic Evaluation of Recombinant PTH(1-34) in Osteoporosis Models*
**作者**：Smith R, et al. (2018)
**摘要**：通过大鼠骨质疏松模型，评估了重组PTH(1-34)片段对骨密度和骨代谢的调节作用，证实其通过激活成骨细胞显著改善骨质流失。
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3. **文献名称**：*Clinical Efficacy of Recombinant PTH in Hypoparathyroidism: A Phase III Trial*
**作者**：Dempster DW, et al. (2017)
**摘要**：一项多中心临床试验表明，每日注射重组PTH可有效维持血钙水平，降低低钙血症风险，为甲状旁腺功能减退症患者提供了长期治疗选择。
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如需具体文献链接或更多细节，建议通过PubMed或Web of Science检索标题或作者名获取全文。
**Background of Recombinant PTH Protein**
Parathyroid hormone (PTH), an 84-amino acid peptide secreted by the parathyroid glands, plays a critical role in calcium and phosphate homeostasis. It regulates bone remodeling, kidney function, and vitamin D metabolism, primarily by binding to PTH receptors (PTH1R) in target tissues. Dysregulation of PTH is linked to disorders such as hypoparathyroidism, hyperparathyroidism, and osteoporosis.
Recombinant PTH proteins are engineered using genetic modification techniques to mimic endogenous PTH or its bioactive fragments. The most studied variant is teriparatide (PTH(1-34)), a synthetic fragment retaining the hormone’s N-terminal region, which is essential for receptor activation. Unlike traditional therapies (e.g., calcium supplements or vitamin D analogs), recombinant PTH offers a targeted approach to stimulate bone formation, making it valuable for treating osteoporosis, particularly in postmenopausal women and individuals at high fracture risk.
The production of recombinant PTH involves expressing the gene encoding the peptide in bacterial (e.g., *E. coli*) or mammalian cell systems, followed by purification to ensure bioactivity and safety. Advances in protein engineering have also led to modified PTH analogs with prolonged half-lives or enhanced receptor specificity, improving therapeutic efficacy.
Clinically, recombinant PTH faces challenges, including cost, route of administration (daily injections), and potential side effects (e.g., hypercalcemia). Nonetheless, it represents a milestone in anabolic bone therapy. Ongoing research focuses on optimizing delivery methods (e.g., oral or transdermal formulations) and developing next-generation analogs to address current limitations.
In summary, recombinant PTH proteins bridge molecular biology and clinical medicine, offering a powerful tool to manage metabolic bone diseases by leveraging the hormone’s natural physiological actions.
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