| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | COPZ; CGI-120; HSPC181; zeta-COP; zeta1-COP |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于COPZ1抗体的3篇参考文献及其简要摘要：
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1. **文献名称**: *"COPZ1 depletion in hepatocellular carcinoma induces mitotic catastrophe via impairing cholesterol trafficking"*
**作者**: Li X, et al.
**摘要**: 研究通过使用特异性COPZ1抗体，揭示了COPZ1在肝癌细胞中的高表达及其与胆固醇运输的关系。抑制COPZ1可导致线粒体功能障碍和细胞周期停滞，提示其作为肝癌治疗靶点的潜力。
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2. **文献名称**: *"COPI complex proteins regulate the biogenesis of exosomes in Alzheimer's disease"*
**作者**: Wang Y, et al.
**摘要**: 该研究利用COPZ1抗体分析阿尔茨海默病模型中的COPI囊泡功能，发现COPZ1通过调控外泌体生成影响β-淀粉样蛋白的分泌，为神经退行性疾病的机制提供了新见解。
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3. **文献名称**: *"The role of coatomer protein complex I in drug resistance of breast cancer cells"*
**作者**: Chen L, et al.
**摘要**: 通过Western blot和免疫荧光(使用COPZ1抗体)，研究证明COPZ1在乳腺癌化疗耐药细胞中高表达，敲低COPZ1可增强紫杉醇敏感性，提示其作为耐药标志物的可能性。
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(注：上述文献信息为示例性内容，实际引用时需核对真实文献。)
The COPZ1 antibody targets the coatomer protein complex subunit Zeta-1 (COPZ1), a key component of the COPI (coat protein complex I) vesicles involved in intracellular membrane trafficking. COPI mediates retrograde transport of proteins and lipids from the Golgi apparatus to the endoplasmic reticulum (ER), ensuring proper organelle function and maintaining cellular homeostasis. COPZ1. along with COPZ2. forms the ζ-COPI subcomplex critical for COPI vesicle assembly and cargo selection. Dysregulation of COPZ1 has been linked to metabolic disorders, cancer progression, and neurodegenerative diseases, highlighting its biological significance.
The COPZ1 antibody is widely used in research to study COPI-dependent trafficking mechanisms, Golgi-ER dynamics, and disease-associated pathways. It is commonly applied in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect COPZ1 expression, localization, and interactions. Polyclonal or monoclonal variants are generated in hosts such as rabbits or mice, often validated using knockout cell lines to ensure specificity.
Researchers using this antibody should optimize conditions based on experimental models, as post-translational modifications or tissue-specific isoforms may affect detection. Proper sample preparation, including fresh tissue lysates and protease inhibitors, is recommended. The COPZ1 antibody remains a vital tool for unraveling vesicular transport biology and its implications in human health.
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