| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DAK; NET45 |
| WB Predicted band size | 59 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human TKFC |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TKFC抗体的示例性参考文献(注：以下文献为模拟示例，非真实存在的论文，供参考)：
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1. **文献名称**：*Development and Validation of a Monoclonal Antibody Against Human Triokinase/FMN Cyclase (TKFC) for Diagnostic Applications*
**作者**：Smith J, et al.
**摘要**：本研究开发了一种针对人源TKFC蛋白的高特异性单克隆抗体，通过免疫印迹和免疫组化验证其灵敏度。该抗体成功应用于肝细胞癌患者的组织样本检测，证实TKFC在肿瘤组织中高表达，提示其作为潜在生物标志物的价值。
2. **文献名称**：*TKFC Antibody-Based Detection of Mitochondrial RNA Processing Defects in Metabolic Disorders*
**作者**：Lee H, et al.
**摘要**：利用TKFC抗体探究其在代谢综合征模型中的表达变化。研究发现，TKFC抗体能有效标记线粒体RNA加工复合体，揭示其在果糖代谢异常和线粒体功能障碍中的调控作用，为代谢疾病机制提供新见解。
3. **文献名称**：*Structural Characterization of TKFC Using Polyclonal Antibodies: Implications for Enzyme Activity Regulation*
**作者**：Garcia R, et al.
**摘要**：通过多克隆抗体分析TKFC蛋白的构效关系，发现特定结构域磷酸化修饰影响其酶活性。该抗体为研究TKFC在先天免疫反应中的功能提供了工具，并支持其在炎症性疾病中的潜在治疗靶点研究。
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如需真实文献，建议在PubMed或Google Scholar中检索关键词“TKFC antibody”或“triokinase antibody”，筛选抗体开发、疾病机制或诊断应用相关研究。
**Background of TKFC Antibody**
The **triokinase/FMN cyclase (TKFC)** antibody targets the TKFC enzyme, a bifunctional protein encoded by the *TKFC* gene in humans. TKFC plays dual roles in cellular metabolism: it functions as a **triokinase** (phosphorylating glyceraldehyde and dihydroxyacetone phosphate in fructose metabolism) and as **FMN cyclase** (catalyzing the conversion of riboflavin-5'-phosphate to cyclic FMN). TKFC is crucial in nucleotide biosynthesis, mitochondrial function, and maintaining cellular redox balance.
Dysregulation of TKFC is linked to metabolic disorders and mitochondrial diseases. Notably, mutations in *TKFC* are associated with **mitochondrial DNA depletion syndrome 7** (MTDPS7), a severe condition characterized by liver dysfunction, neurological deficits, and growth retardation. TKFC also interacts with viral proteins (e.g., hepatitis C virus NS5A), suggesting roles in innate immunity and viral pathogenesis.
TKFC antibodies are essential tools for studying its expression, localization, and interactions in tissues/cells. They are widely used in techniques like Western blotting, immunofluorescence, and ELISA to investigate TKFC's involvement in metabolic diseases, viral infections, and cancer. Research continues to explore TKFC as a potential therapeutic target or biomarker for related pathologies.
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