首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子骨形成蛋白(BMP2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BMP2含量。BMP2（骨形态发生蛋白2）是一种多效性配体蛋白，属于TNFβ家族，参与血管和瓣膜稳态的胚胎发育。它通过结合I型和II型受体调节多种钙化过程，如动脉粥样硬化、慢性肾脏病等。BMP2在骨和软骨的发育中起重要作用，能诱导间充质干细胞分化为成骨细胞和成软骨细胞，促进骨和软骨的生长发育及其重建过程。试剂盒检测范围为1.56 pg/mL-100 pg/mL，该产品适用于骨骼发育机制、组织再生修复、骨代谢相关疾病模型等基础研究，也可用于评估骨生长因子类药物在动物实验中的生物效应。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BMP2 ELISA Kit; BMP-2Bone morphogenetic protein 2 ELISA Kit; BMP-2 ELISA Kit
缩写：BMP2
Uniprot No.：O46564
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit BMP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:2 | Average % | 87 | |||||
| Range % | 80-94 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 85-99 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 85-103 | ||||||
| 1:10 | Average % | 95 | |||||
| Range % | 84-102 | ||||||
回收率：
| The recovery of rabbit BMP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 93 | 82-100 | |||||
| EDTA plasma (n=4) | 97 | 88-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected1002.1362.1772.1572.043501.6121.6281.6201.506251.2211.2391.2301.11612.50.8520.8650.8590.7456.250.5300.5160.5230.4093.120.3510.3650.3580.2441.560.2390.2460.2430.12900.1120.1150.114 | |||||||
货期：3-5 working days
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