首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠骨成型蛋白2(BMP-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的BMP2含量。BMP2 即骨形态发生蛋白 2，是转化生长因子 -β 超家族成员，在胚胎发育、细胞分化及组织修复等过程中起关键作用。其研究机制主要聚焦于通过与受体结合激活下游信号通路，促进骨细胞形成、分化，在骨科疾病治疗等领域有潜在应用价值。试剂盒检测范围为1.56 pg/mL-100 pg/mL，骨再生模型、干细胞定向分化实验、炎症相关骨丢失研究、体外药物筛选、基因编辑模型验证、病理机制研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Bmp2 ELISA Kit; Bmp-2 ELISA Kit; Bone morphogenetic protein 2 ELISA Kit; BMP-2 ELISA Kit; Bone morphogenetic protein 2A ELISA Kit; BMP-2A ELISA Kit
缩写：BMP2
Uniprot No.：P21274
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse BMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 88-101 | |||||
| 1:2 | Average % | 101 | ||||
| Range % | 95-105 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of mouse BMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 80-92 | ||||
| EDTA plasma (n=4) | 101 | 92-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 1002.6432.4532.5482.423 502.0241.9762.0001.875 251.3491.3121.3311.206 12.50.7420.7240.7330.608 6.250.4490.4670.4580.333 3.120.3070.3000.3040.179 1.560.2420.2370.2400.115 00.1260.1240.125 | ||||||
货期：3-5 working days
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