| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HEL-S-98n |
| WB Predicted band size | 30 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human COMT |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于COMT抗体的模拟参考文献示例(内容为虚构，仅作格式参考)：
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1. **文献名称**: *"Specificity Validation of COMT Antibodies in Human and Rodent Brain Tissues"*
**作者**: Smith A, et al.
**摘要**: 本研究通过Western blot和免疫组化技术验证了多种COMT抗体的特异性，发现抗体X在人类及大鼠脑组织中可特异性识别COMT蛋白，未与其他甲基转移酶发生交叉反应，为神经递质代谢研究提供了可靠工具。
2. **文献名称**: *"COMT Protein Expression in Schizophrenia: An Immunohistochemical Analysis"*
**作者**: Chen L, et al.
**摘要**: 利用COMT单克隆抗体对精神分裂症患者前额叶皮层组织进行分析，发现COMT蛋白表达水平与Val158Met基因多态性显著相关，提示其在疾病病理机制中的潜在作用。
3. **文献名称**: *"Localization of COMT in Peripheral Nervous System Using Polyclonal Antibodies"*
**作者**: Garcia R, et al.
**摘要**: 通过多克隆抗体标记，揭示了COMT在周围神经系统的广泛分布，尤其在疼痛信号传导相关区域高表达，支持其在慢性疼痛调控中的功能假说。
4. **文献名称**: *"Impact of Post-Translational Modifications on COMT Antibody Recognition"*
**作者**: Kim H, et al.
**摘要**: 研究证实磷酸化等翻译后修饰可能影响部分COMT抗体的结合效率，建议在蛋白检测实验中优先选用针对非修饰表位的抗体以确保准确性。
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注：以上文献为示例性质，实际引用时需检索真实数据库(如PubMed)并核对原文内容。
Catechol-O-methyltransferase (COMT) antibodies are immunological tools designed to detect and study COMT, an enzyme critical in the metabolism of catecholamine neurotransmitters (e.g., dopamine, epinephrine) and certain drugs. COMT transfers a methyl group to catechol substrates, influencing neurotransmitter inactivation and hormone regulation. It exists in two isoforms: membrane-bound (MB-COMT), predominantly in the brain, and soluble (S-COMT), found in peripheral tissues like the liver. Dysregulation of COMT activity is linked to neurological and psychiatric disorders (e.g., Parkinson’s disease, schizophrenia) due to its role in dopamine degradation, particularly in prefrontal cortex regions affecting cognition and emotion.
COMT antibodies are widely used in research to localize and quantify enzyme expression via techniques like Western blot, immunohistochemistry, and ELISA. They help explore genetic polymorphisms (e.g., Val158Met), which alter enzyme activity and disease susceptibility. Specific antibodies may target distinct epitopes to differentiate isoforms or post-translational modifications. Validation often involves knockout controls or recombinant protein assays to ensure specificity. Additionally, COMT inhibitors (e.g., entacapone) used in Parkinson’s therapy are studied using these antibodies to assess drug effects on enzyme levels. Their applications span neuroscience, pharmacology, and clinical diagnostics, aiding mechanistic insights into COMT-related pathways and therapeutic targeting.
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