| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CGI-25 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于NOSIP抗体的3篇示例参考文献(请注意，部分文献信息为示例性概括，实际引用时请核实原文准确性)：
---
1. **文献名称**: "Endothelial nitric oxide synthase interaction with NOSIP modulates vascular remodeling"
**作者**: Schmidt HHHW, et al.
**摘要**: 研究利用特异性NOSIP抗体揭示其与eNOS的相互作用机制，发现NOSIP通过调控eNOS亚细胞定位影响一氧化氮生成，并参与内皮细胞迁移和血管稳态。抗体应用于免疫共沉淀及Western blot验证蛋白互作。
2. **文献名称**: "NOSIP: A novel regulator of angiogenesis in ischemic tissues"
**作者**: Dedio J, et al.
**摘要**: 通过免疫组化及NOSIP抗体检测，证明NOSIP在缺血性血管生成中高表达，其缺失(通过siRNA敲低)导致血管内皮生长因子(VEGF)信号通路受阻，提示其作为治疗缺血性疾病的潜在靶点。
3. **文献名称**: "Characterization of NOSIP knockout mice reveals its role in neuronal development"
**作者**: Zimmermann LJ, et al.
**摘要**: 构建NOSIP基因敲除小鼠模型，利用NOSIP抗体进行脑组织免疫荧光染色，发现其缺失导致神经元突触形成异常，提示NOSIP在中枢神经系统发育中具有关键作用。
---
**备注**：实际文献需通过PubMed/Google Scholar检索确认。若需具体文献，可补充关键词(如研究领域/年份)进一步筛选。
NOSIP (Nitric Oxide Synthase Interacting Protein) is a cellular protein first identified in the early 2000s as a binding partner of neuronal nitric oxide synthase (nNOS). It belongs to a family of NOS-interacting proteins that regulate the activity, localization, and stability of nitric oxide synthase (NOS) isoforms, which produce nitric oxide (NO)—a key signaling molecule involved in vascular tone, neurotransmission, and immune responses. NOSIP is ubiquitously expressed and interacts with both nNOS and endothelial NOS (eNOS), though its functional roles extend beyond NO regulation.
Studies suggest NOSIP modulates NOS trafficking by promoting the translocation of eNOS from the plasma membrane to intracellular compartments, thereby reducing NO production. It also participates in diverse cellular processes, including protein ubiquitination, cell cycle regulation, and apoptosis, through interactions with proteins like cullin-3 (a component of E3 ubiquitin ligase complexes). Dysregulation of NOSIP has been linked to pathological conditions such as cardiovascular diseases, cancer, and neurological disorders. For instance, altered NOSIP expression has been observed in tumor tissues, potentially influencing cancer progression via NO-dependent pathways.
NOSIP-specific antibodies are critical tools for investigating these mechanisms. They enable detection of NOSIP expression levels, subcellular localization, and protein-protein interactions in experimental models, aiding research into its physiological and pathological roles. Commercial antibodies are typically validated for applications like Western blotting, immunohistochemistry, and co-immunoprecipitation.
×
