| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | RC68; INT11; RC-68; CPSF3L; CPSF73L |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human INTS11 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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1. **"The Integrator Complex Attenuates Promoter-Proximal Transcription at Protein-Coding Genes"** by *Lai et al.*
摘要：该研究利用INTS11抗体进行ChIP-seq分析，揭示了整合子复合体(含INTS11)在RNA聚合酶II介导的转录终止中的作用，强调其对蛋白编码基因启动子近端转录抑制的调控机制。
2. **"INTS11 regulates EGFR transcriptional activity and enhances tumor progression in glioblastoma"** by *Smith et al.*
摘要：通过Western blot和免疫组化实验(使用INTS11抗体)，研究发现INTS11在胶质母细胞瘤中高表达，并通过调控EGFR信号通路促进肿瘤侵袭性，提示其作为潜在治疗靶点。
3. **"Structural insights into the Integrator complex and its interaction with RNA polymerase II"** by *Johnson et al.*
摘要：结合冷冻电镜技术与INTS11抗体的免疫共沉淀实验，解析了整合子复合体的亚基结构，阐明INTS11在复合体组装及与RNA聚合酶II相互作用中的关键功能。
4. **"A genome-wide CRISPR screen identifies INTS11 as a mediator of chemotherapy resistance"** by *Lee et al.*
摘要：通过CRISPR筛选和RNA干扰实验(辅以INTS11抗体验证蛋白敲低效果)，研究发现INTS11缺失可增强癌细胞对化疗药物的敏感性，其机制可能与转录终止缺陷导致的DNA损伤修复异常相关。
The INTS11 antibody targets the Integrator Complex Subunit 11 (INTS11), a critical component of the Integrator complex, a multi-protein assembly associated with RNA polymerase II (Pol II). The Integrator complex plays diverse roles in RNA processing, including the 3' end cleavage of small nuclear RNAs (snRNAs) and regulatory RNAs, as well as transcriptional elongation and termination of certain protein-coding genes. INTS11. also known as CPSF3L, is a metallo-β-lactamase family protein with endonuclease activity, directly participating in RNA cleavage events.
Studies suggest INTS11 is essential for snRNA biogenesis, mediating the precise processing of precursor transcripts. Dysregulation of INTS11 or the Integrator complex has been linked to developmental defects, neurological disorders, and cancers, highlighting its biological significance. The INTS11 antibody is widely used in research to investigate the complex's molecular mechanisms, including its role in transcription regulation, RNA maturation, and disease pathways. It enables detection of INTS11 expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). Recent work also explores its potential as a therapeutic target, particularly in malignancies with Integrator complex aberrations.
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