| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HEN1; C1orf59 |
| WB Predicted band size | 45 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human HENMT1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于HENMT1抗体的3篇代表性文献示例(注：部分内容为假设性概括，实际文献需通过数据库验证)：
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1. **文献名称**：*Structural basis for piRNA-guided silencing of transposable elements by HENMT1*
**作者**：Ipsaro JJ, et al.
**摘要**：本研究解析了HENMT1蛋白的晶体结构，并通过免疫沉淀和Western blot实验验证其与piRNA复合物的相互作用。研究利用特异性HENMT1抗体证明其在生殖细胞中通过甲基化修饰稳定piRNA，抑制转座子活性。
2. **文献名称**：*HENMT1 deficiency disrupts spermatogenesis by impairing piRNA metabolism in mice*
**作者**：Zhang Y, et al.
**摘要**：通过构建HENMT1敲除小鼠模型，研究利用HENMT1抗体进行免疫荧光和蛋白表达分析，发现其缺失导致piRNA 3'端甲基化缺陷，引发精子发育异常和雄性不育。
3. **文献名称**：*Functional characterization of HENMT1 in Drosophila ovarian germline cells*
**作者**：Saito K, et al.
**摘要**：在果蝇卵巢模型中，使用HENMT1抗体进行原位杂交和Western blot检测，揭示HENMT1通过调控piRNA代谢维持生殖细胞基因组稳定性，其缺失导致卵子发生障碍。
4. **文献名称**：*Human HENMT1 mutations cause defective piRNA processing and male infertility*
**作者**：Li X, et al.
**摘要**：临床研究发现人类HENMT1基因突变与无精症相关，通过患者睾丸组织样本的HENMT1抗体染色，证实其蛋白表达异常与piRNA功能失调直接相关。
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**注意**：以上文献标题和作者为示例性内容，实际引用需查询PubMed或SciHub等数据库获取真实文献。建议使用关键词“HENMT1 antibody”、“piRNA HENMT1”进行检索，并优先选择近5年内发表的论文以获取最新进展。
The HENMT1 (Hen Methyltransferase 1) antibody is a tool used to study the HENMT1 protein, a conserved RNA methyltransferase critical for piRNA (PIWI-interacting RNA) biogenesis. HENMT1 catalyzes the 2′-O-methylation of the 3′ termini of piRNAs, a post-transcriptional modification essential for their stability and function in silencing transposable elements and maintaining genome integrity, particularly in germline cells and during early development.
HENMT1 is a key component of the piRNA pathway, and its dysfunction has been linked to infertility, genomic instability, and developmental defects in model organisms. The antibody enables detection and localization of HENMT1 in various experimental settings, such as Western blotting, immunofluorescence, and immunohistochemistry, aiding research on piRNA-mediated gene regulation, epigenetic mechanisms, and reproductive biology.
Developed in hosts like rabbits or mice, HENMT1 antibodies are often validated using knockout controls or enzymatic assays to confirm specificity. Their application spans studies in germ cell development, transposon suppression, and diseases associated with piRNA pathway dysregulation. As piRNA research expands into cancer, neurodegeneration, and aging, HENMT1 antibodies remain vital for elucidating its role in these processes.
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