| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | RVB1; TIH1; ECP54; TIP49; ECP-54; INO80H; NMP238; PONTIN; TIP49A; NMP 238; Pontin52 |
| WB Predicted band size | 50 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human RUVBL1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及RUVBL1抗体的参考文献示例(文献信息基于公开研究归纳，非实时数据库查询)：
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1. **文献名称**: "RUVBL1/RUVBL2 are essential for chromatin remodeling in mammalian stem cell maintenance"
**作者**: J.L. Hartley et al.
**摘要**: 研究利用RUVBL1特异性抗体进行染色质免疫沉淀(ChIP)和Western blot分析，发现RUVBL1/2复合物通过调控染色质重塑维持干细胞多能性，抗体在敲除模型中验证了蛋白表达缺失。
2. **文献名称**: "Structural insights into the role of RUVBL1 in DNA repair complexes"
**作者**: F. Grosse et al.
**摘要**: 通过冷冻电镜解析RUVBL1在DNA修复复合体中的结构，研究中采用RUVBL1抗体进行免疫共沉淀(Co-IP)纯化复合物，并验证其与ATR激酶的相互作用。
3. **文献名称**: "RUVBL1 overexpression promotes tumor progression via mTOR signaling"
**作者**: Y. Wang et al.
**摘要**: 在癌症组织中利用RUVBL1抗体进行免疫组化(IHC)检测，发现其高表达与mTOR通路激活相关，抗体进一步用于体外实验验证靶向干预效果。
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**注意**：以上为模拟示例，实际文献需通过PubMed/Google Scholar检索关键词“RUVBL1 antibody”或“RUVBL1 immunoassay”获取。推荐使用抗体生产商(如CST、Abcam)官网提供的引用文献，通常包含具体应用场景(如WB, IP等)。
RUVBL1 (RuvB-like AAA ATPase 1) is a conserved eukaryotic protein belonging to the AAA+ (ATPases Associated with Diverse Cellular Activities) superfamily. It functions as a helicase and ATP-dependent molecular chaperone, playing critical roles in chromatin remodeling, transcriptional regulation, DNA repair, and cellular signaling. RUVBL1 forms heterohexameric complexes with RUVBL2. serving as a core component of multiple macromolecular assemblies, including the INO80. SWR1. and TIP60 chromatin-remodeling complexes, as well as the mTOR and β-catenin pathways. Its involvement in oncogenic processes, such as cell proliferation and apoptosis evasion, has linked RUVBL1 overexpression to various cancers, making it a potential therapeutic target.
Antibodies targeting RUVBL1 are essential tools for studying its expression, localization, and interactions in normal and pathological contexts. These antibodies are typically raised against specific epitopes, such as the N-terminal or central regions of the human RUVBL1 protein (UniProt ID Q9Y265). Validated applications include Western blotting, immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). High-quality RUVBL1 antibodies exhibit specificity confirmed by knockout/knockdown controls and cross-reactivity with orthologs in common model organisms like mice and rats. Researchers utilize these reagents to explore RUVBL1's role in cancer biology, chromatin dynamics, and ATPase-dependent regulatory mechanisms, as well as to assess its potential as a biomarker or drug target in precision medicine approaches.
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