| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | SEPT10 |
| WB Predicted band size | 53 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human SEPTIN10 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于SEPTIN10抗体的3篇参考文献示例(内容为模拟概括，仅供参考)：
1. **文献名称**：*SEPTIN10参与精子顶体形成的功能研究*
**作者**：Smith A, et al.
**摘要**：通过免疫荧光和Western blot技术，利用SEPTIN10特异性抗体发现其在哺乳动物精子顶体形成阶段高表达，敲除后导致顶体结构异常，表明SEPTIN10对精子形态发生至关重要。
2. **文献名称**：*SEPTIN10在神经元轴突导向中的调控机制*
**作者**：Chen L, et al.
**摘要**：研究采用SEPTIN10抗体进行共聚焦显微成像，揭示其在神经元轴突生长锥中的动态定位，并通过基因沉默证实SEPTIN10通过调控微管稳定性影响轴突导向。
3. **文献名称**：*SEPTIN10与肿瘤细胞迁移的关联分析*
**作者**：Wang Y, et al.
**摘要**：通过免疫组化(SEPTIN10抗体)和体外迁移实验，发现SEPTIN10在乳腺癌细胞中表达上调，抑制其表达可降低细胞侵袭能力，提示其可能作为癌症治疗的潜在靶点。
The SEPTIN10 antibody is a research tool designed to detect and study the SEPTIN10 protein, a member of the septin family involved in diverse cellular processes. Septins are GTP-binding proteins that form filamentous structures, contributing to cytoskeletal organization, cell division, membrane remodeling, and vesicle trafficking. SEPTIN10. specifically, plays roles in spermatogenesis, neuronal development, and cilia function, with emerging links to male infertility, neurodevelopmental disorders, and cancer.
Antibodies targeting SEPTIN10 are typically generated in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. They are widely used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to investigate SEPTIN10's expression patterns, subcellular localization, and interactions in normal and diseased tissues. Commercial SEPTIN10 antibodies are available from suppliers like Abcam, Sigma-Aldrich, and Novus Biologicals, often validated for specificity across human, mouse, or rat models.
Current research leverages these antibodies to explore SEPTIN10's functional aberrations, such as disrupted sperm tail formation or defective cilia in pathological conditions. However, challenges remain, including cross-reactivity with other septin isoforms and variable validation standards. Proper controls (e.g., knockout validation) are critical to ensure antibody reliability. Ongoing studies aim to clarify SEPTIN10's mechanistic roles and therapeutic potential, highlighting its importance in cellular dynamics and disease pathways.
×
