| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | NET59 |
| WB Predicted band size | 63 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human NCLN |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NCLN(Nicalin)抗体的3篇代表性参考文献(内容为模拟虚构，仅供参考)：
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1. **文献名称**：*Nicalin regulates embryonic dorsal-ventral patterning via proteolytic processing of the BMP antagonist Chordin*
**作者**：Matsuo et al.
**摘要**：本研究揭示了NCLN蛋白在脊椎动物胚胎发育中的作用，通过调控Chordin蛋白的切割影响BMP信号通路。利用NCLN特异性抗体进行免疫共沉淀实验，证实其与蛋白酶复合物的相互作用，为胚胎背腹轴形成机制提供新见解。
2. **文献名称**：*Structural and functional analysis of the Nicalin-NOMO protein complex*
**作者**：Haffner et al.
**摘要**：通过冷冻电镜解析NCLN与NOMO蛋白形成的复合物结构，发现其在内质网中的膜蛋白质量控制功能。研究使用NCLN抗体进行亚细胞定位实验，证明该复合物在细胞应激反应中调控未折叠蛋白的降解途径。
3. **文献名称**：*Nicalin modulates apoptosis by interacting with caspase-8 in cancer cells*
**作者**：Zhang et al.
**摘要**：报道NCLN通过结合caspase-8调控肿瘤细胞凋亡。利用NCLN抗体敲低实验证明，NCLN缺失导致caspase-8激活受阻，抑制细胞死亡。研究为靶向NCLN的癌症治疗策略提供了理论基础。
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注：以上文献信息为示例性质，实际研究中请通过PubMed或Google Scholar以关键词"NCLN antibody"或"Nicalin function"检索真实文献。若需具体文献，建议补充NCLN的研究方向(如发育生物学、疾病机制等)。
The Nicalin (NCLN) antibody is a tool used to study Nicalin, an evolutionarily conserved transmembrane protein primarily located in the endoplasmic reticulum (ER). NCLN is part of a protein complex involving TMEM147 and the chaperone protein PGRMC1. which plays a role in ER-associated processes such as protein folding, quality control, and secretion. First identified in zebrafish for its role in embryonic development, NCLN is implicated in modulating signaling pathways, including Nodal/TGF-β signaling, which influences cell differentiation and tissue patterning. Its dysfunction has been linked to developmental defects and diseases like cancer, where altered NCLN expression may affect tumor progression or drug resistance.
The NCLN antibody enables researchers to investigate the protein’s expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and co-immunoprecipitation. Studies using this antibody have shed light on NCLN’s involvement in ER stress responses, apoptosis regulation, and ciliogenesis. Recent research also explores its potential role in neurodegenerative disorders, given the ER’s critical function in neuronal health. Despite its importance, NCLN’s precise molecular mechanisms remain under investigation, emphasizing the continued relevance of specific antibodies in unraveling its biological and pathological functions.
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