Rabbit Polyclonal ATP5F1A Antibody

The ATP5F1A antibody targets the alpha subunit of mitochondrial ATP synthase (Complex V), a key enzyme in oxidative phosphorylation responsible for generating cellular ATP. Encoded by the ATP5F1A gene, this subunit forms the catalytic core of the F1 portion, driving ATP synthesis via proton gradient-driven conformational changes. Dysregulation of ATP5F1A is linked to mitochondrial disorders, neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s), and cancer, where altered energy metabolism is a hallmark. Researchers use ATP5F1A antibodies (monoclonal or polyclonal, often raised in rabbits or mice) to study protein expression, localization, and function in tissues or cultured cells. Common applications include Western blotting, immunohistochemistry, and immunofluorescence. These antibodies are critical for investigating mitochondrial dysfunction, metabolic adaptations in tumors, and therapeutic responses in diseases involving bioenergetic deficits. Validation typically includes knockout controls, species cross-reactivity checks (human, mouse, rat), and concordance with mRNA levels. As ATP synthase is highly conserved, antibody specificity must be rigorously confirmed to avoid cross-reactivity with homologous subunits. ATP5F1A antibodies thus serve as essential tools in unraveling metabolic pathways and disease mechanisms tied to cellular energy homeostasis.