| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 9 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human RPS27L |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于RPS27L抗体的3篇参考文献的简要概括：
1. **文献名称**：*RPS27L promotes TP53-dependent apoptosis and ferroptosis in colorectal cancer*
**作者**：Sasaki M, et al.
**摘要**：该研究揭示了RPS27L在结直肠癌中通过激活p53依赖性凋亡和铁死亡通路抑制肿瘤生长的机制，Western blot实验中使用RPS27L抗体验证其蛋白表达与患者预后负相关。
2. **文献名称**：*RPS27L is a critical regulator of p53 and ribosome biogenesis*
**作者**：Deng Y, et al.
**摘要**：作者发现RPS27L通过结合MDM2调控p53稳定性，并参与核糖体生成。研究通过免疫共沉淀(Co-IP)结合RPS27L抗体，证实其在DNA损伤条件下促进p53介导的细胞周期停滞。
3. **文献名称**：*RPS27L as a potential biomarker for breast cancer progression*
**作者**：Zhang L, et al.
**摘要**：该研究利用RPS27L抗体对乳腺癌组织进行免疫组化分析，发现低表达RPS27L与肿瘤转移和不良预后显著相关，提示其作为治疗靶点的潜力。
4. **文献名称**：*RPS27L modulates the DNA damage response via ubiquitination pathways*
**作者**：Feng H, et al.
**摘要**：研究表明RPS27L通过调控泛素化修饰参与DNA损伤修复，使用RPS27L抗体进行ChIP-seq实验揭示其与特定修复基因启动子区域的结合，影响细胞对化疗的敏感性。
(注：以上文献信息为示例性概括，实际引用需根据具体论文内容调整。)
The RPS27L (Ribosomal Protein S27-like) antibody is a valuable tool for studying the role of the RPS27L protein, a member of the ribosomal protein S27 family. RPS27L shares homology with the small ribosomal subunit protein S27 but has distinct regulatory functions beyond ribosome biogenesis. It is implicated in cellular stress responses, DNA repair, and apoptosis, particularly through its interaction with the tumor suppressor p53. Studies suggest that RPS27L stabilizes p53 by binding to MDM2. a key negative regulator of p53. thereby enhancing p53-mediated transcriptional activity in response to DNA damage. This interaction positions RPS27L as a potential modulator of cell cycle arrest and apoptosis in cancer biology.
The RPS27L antibody is widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect protein expression levels, subcellular localization, and tissue-specific distribution. It aids in exploring RPS27L's differential expression in tumors, its role in chemoresistance, and its prognostic value in cancers such as colorectal and hepatocellular carcinoma. Researchers also utilize this antibody to investigate crosstalk between ribosomal proteins and stress-response pathways. Commercial RPS27L antibodies are typically raised in rabbits or mice, targeting specific epitopes within the protein's conserved regions. Validation includes reactivity assays and knockout cell line controls to ensure specificity. Its applications span basic research on ribosome-mediated signaling and translational studies targeting cancer therapeutics.
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