首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人膀胱肿瘤抗原(BTA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BTA含量。试剂盒检测范围为0.312 ng/mL-20 ng/mL，科研领域可用于膀胱癌发病机制研究、抗肿瘤药物作用效果评估、肿瘤动物模型体液标志物检测等方向，亦可结合组织病理学分析探索BTA在肿瘤微环境中的动态变化规律，为生物标志物筛选和分子诊断技术开发提供实验支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：BTA
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BTA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 87-99 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 95-105 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 86-98 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-102 | |||||
回收率：
| The recovery of human BTA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 87-97 | ||||
| EDTA plasma (n=4) | 93 | 89-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.3442.2892.3172.166101.6491.6221.6361.48551.1291.1211.1250.9742.50.6890.6720.6810.5301.250.4880.4680.4780.3270.6250.3260.3330.3300.1790.3120.2170.2020.2100.05900.1500.1520.151 | ||||||
货期：3-5 working days
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