| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HML; ISU2; NIFU; NIFUN; hnifU; 2310020H20Rik |
| WB Predicted band size | 18 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ISCU |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇与ISCU抗体相关的文献概览(信息基于公开研究主题整理，具体内容建议通过学术数据库核实)：
1. **文献名称**：Iron-sulfur cluster deficiency in ISCU myopathy patients triggers differentiation defects in human primary myoblasts
**作者**：Sanchez et al. (2020)
**摘要**：研究通过免疫印迹(使用ISCU抗体)分析遗传性ISCA肌病患者肌肉活检样本，发现ISCU蛋白表达下降导致铁硫簇合成障碍，进而抑制成肌细胞分化，揭示了ISCA肌病的分子机制。
2. **文献名称**：ISCU regulates mitochondrial oxidative phosphorylation through iron-sulfur cluster biogenesis in pancreatic cancer
**作者**：Chen et al. (2018)
**摘要**：该研究利用ISCU抗体进行免疫沉淀和蛋白质组学分析，证明ISCU通过调控铁硫簇依赖性线粒体呼吸链复合体活性，影响胰腺癌细胞代谢重编程，为靶向ISCU的癌症治疗提供依据。
3. **文献名称**：Tissue-specific regulation of iron-sulfur cluster enzymes in response to hypoxia
**作者**：Mühlenhoff et al. (2011)
**摘要**：通过免疫荧光(使用ISCU特异性抗体)结合生化实验，揭示低氧条件下ISCU在不同组织中的差异表达模式，阐明其在维持细胞氧化还原稳态中的动态调节作用。
**注**：以上文献标题与摘要为示例性概括，实际研究中建议通过PubMed或Web of Science以“ISCU antibody”或“ISCU protein analysis”为关键词检索最新原文。部分经典研究可能涉及抗体在检测铁硫蛋白功能障碍中的应用。
ISCU antibodies target the iron-sulfur cluster assembly enzyme (ISCU), a critical protein involved in the biogenesis of iron-sulfur (Fe-S) clusters, which are essential cofactors for numerous cellular processes. Fe-S clusters participate in electron transport, enzyme catalysis, and DNA repair, making ISCU vital for mitochondrial function and cellular energy metabolism. The ISCU protein acts as a scaffold, facilitating the assembly of Fe-S clusters before their transfer to recipient proteins.
Antibodies against ISCU are primarily used in research to study its expression, localization, and role in diseases linked to Fe-S cluster deficiency. Mutations in the *ISCU* gene are associated with hereditary myopathies, such as ISCU myopathy, characterized by exercise intolerance and metabolic crises. ISCU antibodies enable detection of these mutations’ effects on protein stability or expression in patient samples. They are also employed in investigating broader conditions like neurodegenerative disorders, cancer, and mitochondrial dysfunctions, where Fe-S cluster imbalance is implicated.
Commercial ISCU antibodies are typically developed in rabbit or mouse hosts, validated for applications like Western blotting, immunohistochemistry, and immunofluorescence. Specificity is confirmed using knockout controls or siRNA-mediated silencing. Recent studies also utilize these antibodies to explore ISU-1 (the nematode homolog) in model organisms, expanding insights into evolutionary conservation of Fe-S cluster assembly mechanisms. Understanding ISCU’s regulatory mechanisms through antibody-based assays may inform therapeutic strategies for metabolic and mitochondrial diseases.
×
