| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | L23A; MDA20 |
| WB Predicted band size | 18 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human RPL23A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于RPL23A抗体的参考文献示例(内容基于模拟数据，仅供参考)：
---
1. **文献名称**：*RPL23A modulates apoptosis and cell growth in breast cancer via the p53 pathway*
**作者**：Chen L, et al.
**摘要**：本研究利用RPL23A特异性抗体进行免疫印迹和免疫组化分析，发现RPL23A在乳腺癌组织中高表达，并通过调控p53信号通路影响肿瘤细胞增殖和凋亡。
2. **文献名称**：*Development and characterization of a monoclonal antibody against human RPL23A for ribosome profiling*
**作者**：Smith J, et al.
**摘要**：文章报道了一种新型抗人RPL23A单克隆抗体的制备与验证，该抗体适用于免疫沉淀和超分辨显微镜技术，成功用于解析核糖体组装过程中RPL23A的动态定位。
3. **文献名称**：*RPL23A as a potential biomarker in colorectal cancer: Insights from antibody-based assays*
**作者**：García-Ruiz C, et al.
**摘要**：通过酶联免疫吸附试验(ELISA)和免疫荧光技术，作者发现RPL23A在结直肠癌患者血清中显著上调，提示其作为诊断标志物的潜力。
4. **文献名称**：*RPL23A antibody reveals its essential role in zebrafish embryonic development*
**作者**：Wang Y, et al.
**摘要**：利用RPL23A抗体进行斑马鱼胚胎组织的免疫染色，研究证实RPL23A缺失会导致核糖体生物合成异常，进而引发胚胎发育缺陷。
---
**注**：以上文献为示例，实际引用时请核实真实来源及准确性。建议通过PubMed、Web of Science等平台以关键词“RPL23A antibody”或“RPL23A immunodetection”检索最新研究。
The RPL23A antibody is a crucial tool in studying the ribosomal protein L23a, encoded by the *RPL23A* gene in humans. As a component of the 60S large ribosomal subunit, RPL23A plays a vital role in ribosome biogenesis and protein synthesis. It is evolutionarily conserved, underscoring its fundamental role in cellular function. Research has linked RPL23A to diverse processes, including cell proliferation, differentiation, and stress responses, with dysregulation observed in cancers and genetic disorders.
Antibodies targeting RPL23A are widely used to investigate its expression, localization, and interactions. They enable techniques like Western blotting, immunofluorescence, and immunoprecipitation, aiding in the study of ribosome assembly dynamics or translational regulation. Specific RPL23A antibodies also help explore its non-ribosomal roles, such as potential involvement in p53 signaling or apoptosis.
Commercial RPL23A antibodies are typically raised in hosts like rabbits or mice using peptide antigens or recombinant proteins. Validation includes testing for specificity (e.g., knockdown controls) and cross-reactivity. Reliable antibodies are essential for accurate research, particularly when studying diseases where ribosomal dysfunction is implicated. Ongoing studies continue to clarify RPL23A's multifaceted roles, making its antibody a key reagent in molecular and cellular biology.
×
