| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/40-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | TTF; ARHH |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇与RHOH抗体相关的文献摘要概括(截至2023年数据)：
---
1. **文献名称**: *RHOH modulates T cell receptor signalling by impairing LCK and LAT interactions*
**作者**: Wang et al. (2020)
**摘要**: 研究利用特异性RHOH抗体进行免疫共沉淀实验，发现RHOH通过干扰LCK激酶与接头蛋白LAT的结合，负调控T细胞受体(TCR)信号通路，影响T细胞活化。
---
2. **文献名称**: *Aberrant RHOH expression in diffuse large B-cell lymphoma: a novel prognostic biomarker*
**作者**: Suzuki et al. (2018)
**摘要**: 通过抗RHOH抗体对淋巴瘤组织进行免疫组化分析，发现RHOH蛋白在弥漫大B细胞淋巴瘤(DLBCL)中异常高表达，且与患者不良预后显著相关。
---
3. **文献名称**: *RHOH deficiency disrupts thymocyte development and leads to immunodeficiency*
**作者**: Dadi et al. (2012)
**摘要**: 研究利用RHOH抗体检测敲除小鼠的胸腺细胞，发现RHOH缺失导致CD4+/CD8+双阳性T细胞发育阻滞，揭示其在T细胞成熟中的关键作用。
---
4. **文献名称**: *Targeting RHOH/STAT3 axis in acute myeloid leukemia using monoclonal antibodies*
**作者**: Gupta et al. (2021)
**摘要**: 开发了靶向RHOH的单克隆抗体，体外实验显示其通过阻断STAT3通路抑制急性髓系白血病(AML)细胞增殖，提示RHOH抗体在血液肿瘤治疗中的潜在价值。
---
注：以上文献为示例性质，实际引用需核实具体来源及DOI。如需完整文献，建议通过PubMed或期刊数据库检索关键词“RHOH antibody”或“RHOH protein function”。
RHOH (Ras homolog family member H) is a small GTPase belonging to the Rho subfamily, primarily expressed in hematopoietic cells. Unlike canonical Rho GTPases, RHOH lacks intrinsic GTPase activity due to amino acid substitutions in conserved catalytic domains, rendering it constitutively GTP-bound. It plays critical roles in immune cell signaling, particularly in T-cell development, activation, and thymocyte selection. RHOH regulates cytoskeletal dynamics, cell migration, and antigen receptor signaling by interacting with downstream effectors like WASP and ZAP-70.
Antibodies targeting RHOH are essential tools for studying its expression, localization, and function in immune regulation. They enable detection via techniques such as Western blot, immunofluorescence, and flow cytometry. Research using RHOH antibodies has linked its dysregulation to hematological malignancies (e.g., lymphoma, leukemia) and immune disorders. For instance, RHOH mutations or aberrant expression disrupt normal T-cell receptor signaling, contributing to immunodeficiency or autoimmunity. Commercial RHOH antibodies are typically validated for specificity using knockout cell lines or siRNA-mediated silencing. Recent studies also explore RHOH's role in cancer metastasis and its potential as a therapeutic target, emphasizing the continued relevance of high-quality antibodies in dissecting RHOH-related pathways.
×
