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Human B- and T-lymphocyte attenuator(BTLA) ELISA kit

  • 中文名： 人B和T淋巴细胞衰减因子(BTLA)酶联免疫试剂盒
货号: IPD-EL002868HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：人B和T淋巴细胞衰减因子(BTLA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的BTLA含量。BTLA（B 和 T 淋巴细胞衰减因子）是一种重要靶点。它在免疫调节中起关键作用，可负向调控淋巴细胞的活化与功能。研究机制主要聚焦于其与配体结合后传导抑制性信号，影响免疫细胞的增殖、分化及细胞因子分泌，为免疫相关疾病治疗带来新思路。试剂盒检测范围为0.45 ng/mL-30 ng/mL，适用于科研领域中对BTLA蛋白水平的定量分析，例如在肿瘤微环境研究、免疫细胞功能探索或实验性治疗方案评估中，检测不同病理状态下BTLA的动态变化，为免疫调控机制研究及药物开发提供实验依据。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

  • 别名：B and T lymphocyte associated protein ELISA Kit; B and T lymphocyte attenuator ELISA Kit; B and T lymphocyte associated ELISA Kit; B- and T-lymphocyte attenuator ELISA Kit; B- and T-lymphocyte-associated protein ELISA Kit; BTLA ELISA Kit; BTLA_HUMAN ELISA Kit; BTLA1 ELISA Kit; CD272 ELISA Kit; CD272 antigen ELISA Kit; FLJ16065 ELISA Kit; MGC129743 ELISA Kit

  • 缩写：BTLA

  • Uniprot No.：Q7Z6A9

  • 种属：Homo sapiens (Human)

  • 样本类型：serum, plasma, tissue homogenates

  • 检测范围：0.45 ng/mL-30 ng/mL

  • 灵敏度：0.11 ng/mL

  • 反应时间：1-5h

  • 样本体积：50-100ul

  • 检测波长：450 nm

  • 研究领域：Immunology

  • 测定原理：quantitative

  • 测定方法：Sandwich

  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human BTLA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    		SampleSerum(n=4)
    1:1Average %87
    Range %83-95
    1:2Average %98
    Range %92-103
    1:4Average %108
    Range %102-115
    1:8Average %95
    Range %87-100

  • 回收率：

    The recovery of human BTLA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)9792-102
    EDTA plasma (n=4)9996-106














  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected 302.1672.2472.2072.107 151.5321.4561.4941.394 7.50.9280.9540.9410.841 3.750.5890.5790.5840.484 1.80.3870.4010.3940.294 0.90.2860.2740.2800.180 0.450.1930.1990.1960.096 00.1010.0990.100　 






  • 货期：3-5 working days

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