| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | L18mt; HSPC071; MRP-L18 |
| WB Predicted band size | 21 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human MRPL18 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MRPL18抗体的3篇示例参考文献(内容为虚构示例，仅作格式参考)：
1. **文献名称**：*Mitochondrial Ribosomal Protein L18 (MRPL18) Expression in Colorectal Cancer: A Prognostic Marker*
**作者**：Chen L, et al.
**摘要**：本研究通过免疫组化分析MRPL18在结直肠癌组织中的表达，发现其高表达与患者生存率降低相关，提示MRPL18可能作为癌症进展的生物标志物。
2. **文献名称**：*MRPL18 Regulates Mitochondrial Translation and Apoptosis in Mammalian Cells*
**作者**：Smith J, et al.
**摘要**：利用MRPL18抗体进行Western blot和免疫荧光实验，发现敲低MRPL18会抑制线粒体蛋白质合成并诱导细胞凋亡，表明其在维持线粒体功能中的关键作用。
3. **文献名称**：*Structural Insights into the Assembly of Mitochondrial Ribosomes: Role of MRPL18*
**作者**：Wang Y, et al.
**摘要**：通过免疫沉淀和冷冻电镜技术结合MRPL18抗体，揭示了MRPL18在线粒体核糖体大亚基组装中的结构定位及相互作用网络。
4. **文献名称**：*MRPL18 Mutations Associated with Neurodegenerative Disorders in a Cohort Study*
**作者**：Rodriguez M, et al.
**摘要**：在神经退行性疾病患者中鉴定出MRPL18基因突变，并通过抗体检测发现突变导致其蛋白稳定性下降，提示其与线粒体功能障碍相关。
(注：以上文献为示例，实际引用请通过PubMed、Google Scholar等平台查询真实研究。)
The MRPL18 antibody is a research tool designed to detect mitochondrial ribosomal protein L18 (MRPL18), a key component of the mitochondrial large ribosomal subunit. MRPL18 is encoded by nuclear DNA and plays a critical role in mitochondrial protein synthesis, supporting the assembly and function of oxidative phosphorylation (OXPHOS) complexes essential for ATP production. As mitochondria rely on their own translational machinery to produce subunits of the electron transport chain, MRPL18 is vital for maintaining mitochondrial homeostasis and cellular energy metabolism.
Antibodies targeting MRPL18 are widely used in studies investigating mitochondrial dysfunction linked to diseases such as neurodegenerative disorders, cancer, and metabolic syndromes. They enable the detection of MRPL18 expression levels via techniques like Western blotting, immunofluorescence, and immunohistochemistry, helping researchers assess mitochondrial ribosome integrity and biogenesis. Additionally, MRPL18 antibodies aid in exploring tissue-specific expression patterns or alterations under stress conditions, such as hypoxia or nutrient deprivation.
Commercial MRPL18 antibodies are typically raised in rabbits or mice, with validation data including specificity (e.g., knockdown controls) and cross-reactivity across species (human, mouse, rat). Their applications extend to elucidating MRPL18's regulatory roles in apoptosis, cellular senescence, and disease mechanisms, making them valuable tools in mitochondrial biology and translational research.
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